CCL20, like human β-defensin (hBD)-2, is a potent chemoattractant for CCR6-positive immature dendritic cells and T cells in addition to recently found antimicrobial activities. We previously demonstrated that IL-17 is the most potent cytokine to induce an apical secretion and expression of hBD-2 by human airway epithelial cells, and the induction is JAK/NF-κB-dependent. Similar to hBD-2, IL-17 also induced CCL20 expression, but the nature of the induction has not been elucidated. Compared with a panel of cytokines (IL-1α, 1β, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, IFN-γ, GM-CSF, and TNF-α), IL-17 was as potent as IL-1α, 1β, and TNF-α, with a time- and dose-dependent phenomenon in stimulating CCL20 expression in both well-differentiated primary human and mouse airway epithelial cell culture systems. The stimulation was largely dependent on the treatment of polarized epithelial cultures from the basolateral side with IL-17, achieving an estimated 4- to 10-fold stimulation at both message and protein levels. More than 90% of induced CCL20 secretion was toward the basolateral compartment (23.02 ± 1.11 ng/chamber/day/basolateral vs 1.82 ± 0.82 ng/chamber/day/apical). Actinomycin D experiments revealed that enhanced expression did not occur at mRNA stability. Inhibitor studies showed that enhanced expression was insensitive to inhibitors of JAK/STAT, p38, JNK, and PI3K signaling pathways, but sensitive to inhibitors of MEK1/2 and NF-κB activation, suggesting a MEK/NF-κB-based mechanism. These results suggest that IL-17 can coordinately up-regulate both hBD-2 and CCL20 expressions in airways through differentially JAK-dependent and -independent activations of NF-κB-based transcriptional mechanisms, respectively.
We have recently reported elevated MARCKS phosphorylation (p-MARCKS) in lung cancer tissues but not in their adjacent normal non-cancer tissue sections. We have extended this study to a tumor tissue array from a cohort of 110 human patients with lung cancer. Immunohistochemistry results have shown a significant association of elevated p-MARCKS with advanced-stage, lymph node metastasis and malignant phenotypes of lung cancer, confirming the importance of p-MARCKS in lung cancer progression. To see if this newly discover biomarker can be a target for therapeutic treatment for the suppression of lung cancer malignancy, we have employed two peptides for this potential; one is the MANS peptide developed by our collaborator many years ago to target myristoylated N-terminal amino acid region for the control of airway mucus granule secretion, the other peptide is MPS that directly targets the MARCKS phosphorylation site domain. For MANS peptide, our recent publication has shown it is effective in blocking lung cancer cell migration and invasiveness in vitro as well as in vivo metastasis. A similar observation was seen for MPS peptide treatment. However, in contrast to MANS peptide, MPS treatment can directly block PIP3-dependent AKT signaling and repress lung tumor growth in vivo in addition to the suppression of metastasis, while MANS can only reduce cancer metastasis. These results support the initial notion on the involvement of p-MARCKS as a biomarker in lung cancer and the potential to target the phosphorylation site domain for controlling cancer progression. Our data have also suggested a direct targeting MARCKS phosphorylation site domain by MPS peptide is more effective in the suppression of lung cancer malignancy. Citation Format: Ching-Hsien Chen, Sarah Statt, Phillip Thai, Jasmine G. Lee, Kenneth B. Adler, Pan-Chyr Yang, Reen Wu. Suppression of lung cancer malignancy by peptides targeting MARCKS phosphorylation site domain: A comparative study. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 2596. doi:10.1158/1538-7445.AM2014-2596
Myristoylated alanine-rich C kinase substrate (MARCKS), a substrate of protein kinase C (PKC), is a key regulatory molecule controlling mucin secretion by airway epithelial cells in vitro and in vivo. Phosphorylated MARCKS (p-MARCKS) has been reportedly to promote inflammatory cell migration in several lung diseases. However, the functionality of MARCKS and its related phosphorylation in lung cancer malignancy has not been characterized. This study demonstrated MARCKS expression and elevated p-MARCKS in various lung cancer cell lines with high malignancy, such as CL1-0-F3, CL1-5, PC9, A549 and several tyrosine kinase inhibitor (TKI) resistant cell lines (H1650 and H1975), as compared with normal human bronchial epithelial (NHBE) cells and low metastasis cancer cell line. siRNA knockdown MARCKS expression resulted in an inhibition of cell migration, accompanying with a decrease of Slug expression in these malignant lung cancer cell lines. Treatment with myristoylated 24-amino-acid MARCKS N-terminus sequence (MANS) peptide, but not the control myristoylated random N-terminal sequence (RNS) peptide, suppressed MARCKS phosphorylation and impaired cell migration and wound healing ability in these lung cancer cell lines. Importantly, MANS peptide treatment resulted in a suppression of lamellipodia/filopodia formation and an enhancement of cell-cell contacts with increased E-cadherin expression. These results confirmed a crucial role for MARCKS, specifically its phosphorylation, in potentiating lung cancer cell migration and malignancy. This study has also suggested a potential usage of MANS or the MARCKS-related peptides in the treatment of lung cancer metastasis. Citation Format: Ching-Hsien Chen, Phillip Thai, Reen Wu. Myristoylated alanine-rich C kinase substrate (MARCKS) phosphorylation potentiates human lung cancer cell malignancy . [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4919. doi:10.1158/1538-7445.AM2013-4919
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