As keratinocytes were derived from epidermal stem cells of the hair follicles and were obtained from nonlesional sites, differences found are likely to present an intrinsic feature of psoriasis epithelium. Our data support the significance of IL-1 family members as therapeutic targets in psoriasis conditions.
Additional Supporting Information may be found in the online version of this article:Data S1. Methods . Table S1. The distribution and Cox survival analysis of the genotype and haplotype of two VDBP SNPs between BCC patients and the reference group. Table S2. Association between the genotype and haplotype of two VDBP SNPs and the development of a first BCC stratified for age at study entry. Abstract: Keratinocytes and activated T cells interact in skin inflammation by virtue of chemokines and cytokines. T cellderived IL-17 has been described to play an important role in the course of psoriatic inflammation. In this study, we addressed how keratinocytes influence the secretion of IL-17 in autologous T cell subsets. We found that a co-culture of autologous keratinocytes and T cell-receptor-stimulated T cells markedly enhanced the production of IL-17. Besides the importance of direct cell contact, this effect was mainly mediated by IL-1 and could be blocked by the IL-1 antagonist anakinra. An additional increase in IL-17 production by IL-23 was only seen in the presence of IL-1, which thus plays a permissive role for the action of IL-23. Importantly, co-culture of keratinocytes with CCR6+ CD4+ T cells that are enriched for Th17 cells resulted in significantly higher IL-17 production compared to co-culture with CD4+ T cells.
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