Pogang gan (Curcuma sp., Zingiberaceae family) is medicinal herb and threatened and it is usually used as a tonic. We successfully micropropagated and cultivated this species. This study determined the total alkaloid, polyphenol, flavonoid and saponin contents of its growth time in order to determine harvest time. The highest level of total alkaloid content is roots after 11 months cultivation (0.272 mg/g), total polyphenol content is rhizomes after 11 months (1.71 mg GAE/g), total flavonoid content is rhizomes after 11 months (13.89 mg QE/g) and total saponin content is tubers and roots after 11 months (5.26% and 4.78%). The total content of alkaloid and flavonoid of pogang gan is higher than Curcuma zedoaria. The total saponin content of natural pogang gan is equal to 39.5% of 6-year old Vietnamese ginseng and the saponin content in rhizomes and tubers of 11-month-planted Pogang gan is from 55.5% to 67.8%, compared with natural Pogang gan.
A rapid and improved micropropagation protocol was developed for Curcuma sp., a threatened and high value medicinal plant, using main bud sprout and top of rhizome sprout as explants. Stepwise optimization of different plant is to change the growth regulator, reduce the level of macro-nutrition and add humate. The present study has created multiple shoot and root induction and plantlet in vitro culture, transfers the plantlet to ex vitro. The M2 medium (MS's macronutrition ¼, MS's micronutrition + Morel's vitamine + coconut milk 10% + sucrose 25 g/l + humate 1.0 ml/l + agar 7.5 g/l + 2,4D 0.5 mg/l + BAP1.0 mg/l + TDZ 1.0 mg/l) is the highest ratio of callus induction. The TA3 medium (MS's macronutrition ¼ + MS's micronutrition + Morel's vitamin+ coconut milk 10% + sucrose 25g/l + humate 2.0 ml/l + 2,4 D 0.5 mg/l + Kinetin 2.0 mg/l + TDZ 1.0 mg/l + BAP 1.0 mg/l2 + NAA 2.0 mg/l + activated carbon 2.0 g/l) is able to create buds and regeneration multiple bud for Curcuma sp. TA3 medium adding IAA 2 mg/l and IBA 0.5 mg/l has resulted in the highest indices of quantity, healthy shoot and large diameter of roots. A large number of healthy plantlets are induced by the medium of MS's macronutrients ½, MS's micronutrients full, Morel's vitamin, humate 3 g/l, coconut milk 150 ml/l, activated carbon 3 g/l, composition phytohormone: IAA 2 mg/l + BAP 2 mg/l + TDZ 0.5 mg/l. Further studies should focus on optimizing the humate concentration for these species of Zingiberaceae. The duration of the research is from 5/2015 to 4/2016 at Faculty of Agriculture and Forestry, Tay Nguyen University, Vietnam.
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