Background:
Oleuropein is a potent antioxidant and free-radical scavenger with antiinflammatory
properties.
Objectives:
In the present study, we evaluated the protective effects of oleuropein on
myeloperoxidase (MPO) activity, nitrite, urea, creatinine and glomerulosclerosis in
alloxan-induced type 1 diabetic rats.
Materials and Methods:
Thirty Sprague-Dawley male rats were randomly divided into 3
groups: group 1 as control; group 2 as untreated diabetic; and group 3 as treated with
oleuropein 15 mg/kg i.p daily. Diabetes was induced in the second and third groups by
subcutaneous alloxan injection. After 48 days, the animals were anaesthetized and then
the livers and kidneys were removed immediately and used fresh or kept frozen until MPO
activity analysis. Blood samples were also collected before sacrificing to measure nitrite,
urea, and creatinine. Kidney paraffin sections were prepared to estimate glomerular
volume, leukocyte infiltration, and glomerulosclerosis.
Results:
Oleuropein significantly decreased leukocyte infiltration and glomerulosclerosis in
the treated group compared with the diabetic untreated group. Oleuropein significantly
decreased the levels of urea, nitrite, and creatinine in the treated group compared with the
diabetic untreated group. Moreover, oleuropein significantly decreased MPO activity in
the treated group compared with the diabetic untreated group.
Conclusions:
Oleuropein has antioxidative and antiatherogenic activities and exerts beneficial
effects on inflammation and kidney function test and decreases diabetic complication in
diabetic rats.
BackgroundThere is increasing evidence indicating an aberrant expression of miRNAs in colorectal cancer (CRC) development. Growing evidence has suggested that polyunsaturated fatty acids (PUFAs) could modulate the remodeling of the epigenome. No study has yet been published to examine the direct effect of PUFA on the promoter methylation of miRNAs. This study aimed to examine the potential clinical application of PUFA on the promoter DNA methylation of miR-126 and its angiogenic target molecule (VEGF) in the CRC cells.MethodsWe investigated the direct effect of 100 μM EPA, DHA, and LA for 24 h on promoter methylation status of miR-126 in a panel of five CRC cell lines (HCT116, HT29/219, Caco2, SW742, and LS180) by methylation-specific PCR (MSP). We also quantified the miR-126 and VEGF transcript expression levels in five CRC cell lines affected by PUFA by real-time PCR. Moreover, we analyzed the protein expression level of VEGF, as a target of miR-126, by western blotting assay.ResultsMSP analysis showed extensive DNA methylation of the miR-126 promoter in all five CRC cell lines, and among all three PUFAs, only DHA completely demethylated the promoter of miR-126 in HCT116 and Caco2 cell lines. We found that only DHA significantly induces the expression level of miR-126 in HCT116 and Caco2 cell lines, respectively, by 20.1-fold and 1.68-fold (p < 0.05). Our finding indicates that the downregulation of VEGF protein level is also effectively observed only in DHA-treated HCT116 and Caco2 cells compared to control cells (p < 0.05).ConclusionsOur results provide evidence that n-3 PUFAs are able to modulate cellular miR-126 DNA methylation and inhibit VEGF expression level in a cell-type specific manner in colorectal cancer cells. DHA always showed higher efficacy than EPA and LA in our experiment. Overall, our results suggest a potential clinical application of n-3 PUFAs as anti-angiogenic agents in CRC therapy.
Background: Nephrotoxicity is one of the most important side effects of the use of gentamicin sulphate (GS) resulted in reactive oxygen species generation. Antioxidant compounds played effective roles in reduction of renal injuries caused by using of gentamicin. Carvacrol is a strong antioxidant compound. Objectives: The aim of this study is to explore the effect of carvacrol inhibition in lesions of gentamicin-induced nephrotoxicity.
Materials and Methods:In this experimental study, 32 male mature Sprague-Dawley rats were divided into 4 groups of 8; group1: control, group 2 sham received daily carvacrol injection (74 mg/kg) for 12 days, group 3 received daily GS injection (100 mg/kg) for 12 days, group 4 received daily GS (100 mg/kg) and carvacrol (74 mg/kg) for 12 days. After 12 days, rats were anaesthetized, blood sample were obtained and kidneys were removed then stained with hematoxylin and eosin method and then were studied histophatologically. Serum creatinine and urea were measured. Results: Flow treatment of nephrotoxic animals with carvacrol could significantly inhibit leukocyte infiltration (9.42%) and tubular necrosis (38.18%) in comparison with the nephrotoxic untreated group. Carvacrol significantly decreased the levels of urea and creatinine in treated group compared with the nephrotoxic untreated group. Conclusions: The findings showed that carvacrol alleviates loss of leukocyte infiltration (9.42%) and tubular necrosis and exerts beneficial effects on kidney function test in nephrotoxic group.
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