The DMSO lanes in Fig. 2C and D were duplicated in Fig. 2E and F, respectively, which contravenes our image presentation guidelines. The authors supplied full blots for all these panels and prepared a new figure using these. The corrected and original panels are shown below. Both the online full-text and PDF versions of the article have been updated. Fig. 2 (corrected panels). M-α fragments accumulate upon interference with PIKfyve function or lysosomal protease activity. (C,D) MNT-1 cells were treated for 2 h or 24 h with 1.6 µM YM201636 or with a mixture of protease inhibitors (100 µM leupeptin, 10 µM pepstatin A and 10 µM E-64d) and Triton X-100-soluble (C) and Triton X-100-insoluble (D) lysates were analyzed by immunoblotting using antibodies against the PMEL C-terminus (anti-PMEL-C), the PMEL N-terminus (anti-PMEL-N), the PMEL RPT domain (anti-PMEL-HMB45), the PMEL PKD domain (anti-PMEL-I51) and tubulin (anti-TUB), as an equal loading marker. The different PMEL fragments are annotated on the right. Stars indicate M-α fragments derived from another isoform generated by alternative splicing. Right-hand panels show higher exposures.
Intersectins (ITSNs) are a family of highly conserved proteins with orthologs from nematodes to mammals. In vertebrates, ITSNs are encoded by two genes (itsn1 and itsn2), which act as scaffolds that were initially discovered as proteins involved in endocytosis. Further investigation demonstrated that ITSN1 is also implicated in several other processes including regulated exocytosis, thereby suggesting a role for ITSN1 in the coupling between exocytosis and endocytosis in excitatory cells. Despite a high degree of conservation amongst orthologs, ITSN function is not so well preserved as they have acquired new properties during evolution. In this review, we will discuss the role of ITSN1 and its orthologs in exo- and endocytosis, in particular in neurons and neuroendocrine cells.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.