Corneal evaluation in ophthalmology necessitates cellular-resolution and fast imaging techniques allowing accurate diagnoses. Currently, the fastest volumetric imaging technique is Fourier-domain full-field optical coherence tomography (FD-FF-OCT) that uses a fast camera and a rapidly tunable laser source. Here, we demonstrate high-resolution, highspeed, non-contact corneal volumetric imaging in vivo with FD-FF-OCT that can acquire a single 3D volume with a voxel rate of 7.8 GHz. The spatial coherence of the laser source was suppressed to prevent it from focusing to a spot on the retina, and therefore, exceeding the maximum permissible exposure (MPE). Inherently volumetric nature of FD-FF-OCT data enabled flattening of curved corneal layers. Acquired FD-FF-OCT images revealed corneal cellular structures, such as epithelium, stroma and endothelium, as well as subbasal and midstromal nerves.
We present in-vivo imaging of the mouse brain using custom made Gaussian beam optical coherence microscopy (OCM) with 800nm wavelength. We applied new instrumentation to longitudinal imaging of the glioblastoma (GBM) tumor microvasculature in the mouse brain. We have introduced new morphometric biomarkers that enable quantitative analysis of the development of GBM. We confirmed quantitatively an intensive angiogenesis in the tumor area between 3 and 14 days after GBM cells injection confirmed by considerably increased of morphometric parameters. Moreover, the OCM setup revealed heterogeneity and abnormality of newly formed vessels.
OCT-A is becoming more popular in recent years and there is a high demand to improve the quality of angiograms as well as to extract quantitative information. We applied various processing methods for microvasculature enhancement like Hessian-Frangi to a data set obtained with Bessel and Gaussian OCT systems. We used angiogenesis, fractal and multifractal analysis to extract more quantitative information. We applied the processing methods for healthy, stroke, tumor progression and the results of enhanced processing and quantitative analysis for those are presented in this letter. Full Text: PDF ReferencesJ. G. Fujimoto, C. Pitris, S. A. Boppart, and M. E. Brezinski, "Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos", Neoplasia (New York, NY), 2000, 2(1-2):9-25 CrossRef O. Liba, E. D. SoRelle, D. Sen, and A. de la Zerda, "Contrast-enhanced optical coherence tomography with picomolar sensitivity for functional in vivo imaging", Sci Rep. 2016, 6(1):23337. CrossRef O Liba, M. D. Lew, E. D. SoRelle, et al., "Speckle-modulating optical coherence tomography in living mice and humans", Nat Commun. 2017, 8:15845. CrossRef K. Karnowski, A. Ajduk, B. Wieloch, et al., "Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos", Sci Rep. 2017, 7(1):4165. CrossRef V. J. Srinivasan, S. Sakadžić, I. Gorczynska, et al., "Quantitative cerebral blood flow with Optical Coherence Tomography", Opt Express. 2010, 18(3):2477. CrossRef S. Tamborski, H. C. Lyu, H. Dolezyczek, et al. Extended-focus optical coherence microscopy for high-resolution imaging of the murine brain. Biomed Opt Express. 2016, 7(11):4400-4414. CrossRef A. F. Frangi, W. J. Niessen, K. L. Vincken, and M. A. Viergever, "Multiscale vessel enhancement filtering", Lect Notes Comput Sc. 1998;1496:130–137 CrossRef A. A. Ucuzian, A. A. Gassman, A. T. East, and H. P. Greisler , Journal of burn care & research: official publication of the American Burn Association 2010 , 31(1):158. CrossRef R. Lopes, and Betrouni, N. (2009). "Fractal and multifractal analysis: A review", Med. Image Anal. 13, 634–649. CrossRef J. W. Baish and R. K. Jain., "Correspondence re: J. W. Baish and R. K. Jain, Fractals and Cancer. Cancer Res., 60: 3683-3688, 2000.", Cancer Res. 2000, Jul 15, 60(14):3683-8. DirectLink
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