Wide-ranging large carnivores pose myriad challenges for conservation, especially in highly fragmented landscapes. Over a 13-year period, we combined monitoring of radio collared pumas (Puma concolor) with complementary multi-generational genetic analyses to inform puma conservation in southern California, USA. Our goals were to generate survivorship estimates, determine causes of mortality, identify barriers to movement, and determine the genetic and demographic challenges to puma persistence among >20,000,000 people and extensive urban, suburban, and exurban development. Despite protection from hunting, annual survival for radio collared pumas was surprisingly low (55.8%), and humans caused the majority of puma deaths. The most common sources of mortality were vehicle collisions (28% of deaths), and mortalities resulting from depredation permits issued after pumas killed domestic animals (17% of deaths). Other human-caused mortalities included illegal shootings, public safety removals, and human-caused wildfire. An interstate highway (I-15) bisecting this study area, and associated development, have created a nearly impermeable barrier to puma movements, resulting in severe genetic restriction and demographic isolation of the small puma population (n ~ 17–27 adults) in the Santa Ana Mountains west of I-15. Highways that bisect habitat or divide remaining “conserved” habitat, and associated ongoing development, threaten to further subdivide this already fragmented puma population and increase threats to survival. This study highlights the importance of combining demographic and genetic analyses, and illustrates that in the absence of effective measures to reduce mortality and enhance safe movement across highways, translocation of pumas, such as was done with the endangered Florida panther (P. c. coryi), may ultimately be necessary to prevent further genetic decline and ensure persistence of the Santa Ana Mountains population.

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Although a plethora of habitat-connectivity plans exists, protecting and restoring connectivity through on-the-ground action has been slow. We identified challenges to and opportunities for connectivity conservation through a literature review of project implementation, a workshop with scientists and conservation practitioners, 3 case studies of connectivity projects, and interviews with conservation professionals. Connectivity challenges and solutions tended to be context specific, dependent on land-ownership patterns, socioeconomic factors, and the policy framework. Successful connectivity implementation tended to be associated with development and promotion of a common vision among diverse sets of stakeholders, including nontraditional conservation actors, such as water districts and recreation departments, and with communication with partners and the public. Other factors that lead to successful implementation included undertaking empirical studies to prioritize and validate corridors and the identification of related co-benefits of corridor projects. Engaging partners involved in land management and planning, such as nongovernmental conservation organizations, public agencies, and private landowners, is critical to effective strategy implementation. A clear regulatory framework, including unambiguous connectivity conservation mandates, would increase public resource allocation, and incentive programs are needed to promote private sector engagement. Connectivity conservation must move more rapidly from planning to implementation. We provide an evidence-based solution composed of key elements for successful on-the-ground connectivity implementation. We identified the social processes necessary to advance habitat connectivity for biodiversity conservation and resilient landscapes under climate change.

Wide-ranging large carnivores pose myriad challenges for conservation, especially in highly fragmented landscapes.Over a 13-year period, we combined monitoring of radio collared pumas with complementary multi-generational genetic analyses to inform puma conservation in southern California. Our goals were to generate survivorship estimates, determine causes of mortality, identify barriers to movement, and determine the genetic and demographic challenges to puma persistence among >20,000,000 people and extensive urban, suburban, and exurban development. Despite protection from hunting, annual survival for radio collared pumas was surprisingly low (55.8%), and humans caused the majority of puma deaths. The most common sources of mortality were vehicle collisions (28% of deaths), and mortalities resulting from depredation permits issued after pumas killed domestic animals (17% of deaths). Other human-caused mortalities included illegal shootings, public safety removals, and human-caused wildfire. An interstate highway (I-15) bisecting this study area, and associated development, have created a nearly impermeable barrier to puma movements, resulting in severe genetic restriction and demographic isolation of the small puma population (~17-27 adults) in the Santa Ana Mountains west of I-15. Highways that bisect habitat or divide remaining "conserved" habitat, and associated ongoing development, threaten to further subdivide this already fragmented puma population and increase threats to survival. This study highlights the importance of combining demographic and genetic analyses, and illustrates that in the absence of effective measures to reduce mortality and enhance safe movement across highways, translocation of pumas, such as was done with the endangered Florida panther, may ultimately be necessary to prevent further genetic decline and ensure persistence of the Santa Ana Mountains population.