Baricitinib, is an oral Janus kinase (JAK)1/JAK2 inhibitor approved for the treatment of rheumatoid arthritis (RA) that was independently hypothesized, using artificial intelligence (AI)-algorithms, to be useful for the treatment of COVID-19 infection via a proposed anti-cytokine effects and as an inhibitor of host cell viral propagation 1,2. We validated the AI-predicted biochemical inhibitory effects of baricitinib on human numb-associated kinase (hNAK) members measuring nanomolar affinities for AAK1, BIKE, and GAK. Inhibition of NAKs led to reduced viral infectivity with baricitinib using human primary liver spheroids, which express hAAK1 and hGAK. We evaluated the in vitro pharmacology of baricitinib across relevant leukocyte subpopulations coupled to its in vivo pharmacokinetics and showed it inhibited signaling of cytokines implicated in COVID-19 infection. In a case series of patients with bilateral COVID-19 pneumonia, baricitinib treatment was associated with clinical and radiologic recovery, a rapid decline in SARS-CoV-2 viral load, inflammatory markers, and IL-6 levels. This represents an important example of an AI-predicted treatment showing scientific and clinical promise during a global health crisis. Collectively, these data support further evaluation of the AI-derived hypothesis on anti-cytokine and anti-viral activity and supports its assessment in randomized trials in hospitalized COVID-19 patients.

The generation of organoids is one of the biggest scientific advances in regenerative medicine. Here, by lengthening the time that human pluripotent stem cells (hPSCs) were exposed to a three-dimensional microenvironment, and by applying defined renal inductive signals, we generated kidney organoids that transcriptomically matched second-trimester human fetal kidneys. We validated these results using ex vivo and in vitro assays that model renal development. Furthermore, we developed a transplantation method that utilizes the chick chorioallantoic membrane. This approach created a soft in vivo microenvironment that promoted the growth and differentiation of implanted kidney organoids, as well as providing a vascular component. The stiffness of the in ovo chorioallantoic membrane microenvironment was recapitulated in vitro by fabricating compliant hydrogels. These biomaterials promoted the efficient generation of renal vesicles and nephron structures, demonstrating that a soft environment accelerates the differentiation of hPSC-derived kidney organoids.

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Epidemiological aspects of human and canine visceral leishmaniasis inNinety-five cases of human VL were reported in the period. The 0-9-year-old age group (48.4%) was the most affected, within which the majority consisted of male patients (64%). Of the samples collected for the canine serological survey, 2,919 (6.3%) were reactive to VL. The spatial localization of these cases shows that the disease was scattered in the urban area of the municipality. Areas showing a higher dissemination risk were concentrated in the central, northwestern, and southern regions of the city. Conclusions: Identifying the areas most at risk in urban Montes Claros may help guide actions toward local epidemiological vigilance and control.

Objective: To identify in the literature the defining characteristics and related factors of the nursing diagnosis "ineffective breathing pattern". Method: Integrative review with the steps: problem identification, literature search, evaluation and analysis of data and presentation of results. Results: Twenty articles and two dissertations were included. In children, the most prevalent related factor was bronchial secretion, followed by hyperventilation. The main defining characteristics were dyspnea, tachypnea, cough, use of accessory muscles to breathe, orthopnea and adventitious breath sounds. Bronchial secretion, cough and adventitious breath sounds are not included in the NANDA-International (NANDA-I). For adults and older adults, the related factors were fatigue, pain and obesity and the defining characteristics were dyspnea, orthopnea and tachypnea. Conclusion: This diagnosis manifests differently according to the patients’ age group. It was observed that some defining characteristics and related factors are not included in the NANDA-I. Their inclusion can improve this nursing diagnosis.

OBJETIVO: Descrever as características clínicas e epidemiológicas e o tratamento das crianças internadas com leishmaniose visceral (LV) em hospital universitário de referência no norte de Minas Gerais, Brasil. MÉTODOS: Estudo retrospectivo e documental de crianças de 0 a 12 anos internadas com diagnóstico de LV no Hospital Universitário Clemente de Faria, Montes Claros, MG. Foram analisados os prontuários referentes ao período de janeiro de 2006 a dezembro de 2007. RESULTADOS: Foram identificadas 51 crianças com LV, sendo 51% do sexo feminino e a faixa etária de maior incidência da doença foi em menores de 5 anos (74,5% dos casos). Verificou-se que 31% dessas crianças residiam em Montes Claros e 69% procediam de 20 municípios do norte de Minas Gerais, dos quais 72,5% eram originárias da zona urbana e 21,6% da zona rural. A principal manifestação clínica foi a febre (96,1%) e os principais achados clínicos na admissão foram esplenomegalia (98%) e hepatomegalia (94%). O tratamento de escolha foi Glucantime (70,6%), Anfotericina B convencional (13,7%), Anfotericina B lipossomal (2%) e Glucantime associado a Anfotericina B (15,7%). 35,4% dos casos desenvolveram processos infecciosos durante o período da internação, destacando-se as infecções de pele em 11,8%. O tempo de permanência hospitalar médio foi de 19 dias (DP = ±5,4), 96,1% receberam alta médica e 3,9% evoluíram para óbito. CONCLUSÕES: A partir das características clínicas e epidemiológicas identificadas no estudo, sugere-se uma observação mais eficaz por parte dos profissionais de saúde, visando ao reconhecimento precoce e tratamento adequado da doença e suas complicações.

RESUMO:Objetivo: construir e validar o conteúdo de um instrumento para registro da assistência de Enfermagem Perioperatória para um Hospital de Clínicas, visando a atender as exigências da Organização Mundial da Saúde propostas no 2.º Desafio Global e reafirmadas no Manual Cirurgias Seguras Salvam Vidas do Ministério da Saúde Brasileiro. Método: trata-se de um relato de experiência. A construção do instrumento fundamentou-se na Teoria das Necessidades Humanas Básicas e na revisão bibliográfica sobre o tema. Para a validação de conteúdo, foram realizadas aulas expositivas para treinamento da Equipe de Enfermagem. Resultados: o instrumento foi analisado como satisfatório pelas avaliadoras por ser de fácil interpretação e preenchimento. Conclusão: ressalta-se a importância da parceria entre a academia e o serviço de saúde. Sugere-se que o desenvolvimento de instrumentos com esta finalidade seja realizado em conjunto, no intuito de auxiliar os profissionais nos campos de estágio, envolver alunos estagiários e valorizar a assistência de Enfermagem. PALAVRAS-CHAVE:Enfermagem perioperatória. Diagnósticos de enfermagem. Segurança.ABSTRACT: Objective: The purpose of this study is to develop and validate an instrument of perioperative nursing for a university hospital aiming to meet the requirements proposed at the 2nd Global Challenge of the World Health Organization (WHO) and reiterated in the "Safe Surgeries Save Lives" Manual from the Brazilian Ministry of Health. Method: It is an experience report of descriptive approach. The construction of the instrument was based on the Basic Human Needs Theory and on literature review. Training lectures were conducted with the nursing staff to validate the instrument contents. Results: The instrument was assessed as satisfactory by evaluators because it can be easily filled in. Conclusion: We realized how important the partnership between the academy and the health service is. We suggest that more studies such as this one be undertaken in order to assist the field of training, engage trainee students, and enable the recovery of our profession.KEYWORDS: Perioperative nursing. Nursing diagnosis. Safety. RESUMEN:Objetivo: construir y validar el contenido de un instrumento para registro de la asistencia de enfermería perioperatoria en un Hospital de Clínicas, con el objetivo de atender las exigencias de la Organización Mundial de Salud, propuestas en el 2º Desafío Global y reafirmadas en el Manual "Cirugías Seguras Salvan Vidas" del Ministerio de Salud. Método: se trata de un relato de experiencia. La construcción del instrumento se fundamentó en la teoría de las Necesidades Humanas Básicas y en la revisión bibliográfica sobre el tema. Para la validación del contenido se realizaron clases expositivas, con el fin de entrenar al equipo de enfermería. Resultados: el instrumento fue evaluado como satisfactorio al ser de fácil interpretación y cumplimentación. Conclusión: se resalta la importancia de que haya colaboración entre el mundo académico y el servicio de salud. Se sugiere que el ...