Aims: Urinary 8-oxo-7,8-dihydro-2¢-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical-and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject-and sample collection-related variables, as potential sources of variability. Results: Chromatographic assays showed high agreement across urines from different subjects, whereas ELISAs Kronos Science, Phoenix, Arizona. ANTIOXIDANTS & REDOX SIGNALINGVolume 18, Number 18, 2013 ª Mary Ann Liebert, Inc. DOI: 10.1089/ars.2012.4714 2377showed far more inter-laboratory variation and generally overestimated levels, compared to the chromatographic assays. Excretion rates in timed 'spot' samples showed strong correlations with 24 h excretion (the 'gold' standard) of urinary 8-oxodG (r p 0.67-0.90), although the associations were weaker for 8-oxodG adjusted for creatinine or specific gravity (SG). The within-individual excretion of 8-oxodG varied only moderately between days (CV 17% for 24 h excretion and 20% for first void, creatinine-corrected samples). Innovation: This is the first comprehensive study of both human and methodological factors influencing 8-oxodG measurement, providing key information for future studies with this important biomarker. Conclusion: ELISA variability is greater than chromatographic assay variability, and cannot determine absolute levels of 8-oxodG. Use of standardized calibrants greatly improves intra-technique agreement and, for the chromatographic assays, importantly allows integration of results for pooled analyses. If 24 h samples are not feasible, creatinine-or SG-adjusted first morning samples are recommended.
Running Title: Measurement of anandamide in human plasma Abbreviations: AEA, N-arachidonoylethanolamine (anandamide); AEA-d8, octo-deuterated anandamide; LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation; UPLC, ultra performance liquid chromatography, HPLC, high performance liquid chromatography; MS/MS, tandem mass spectrometry Keywords: anandamide, endocannabinoid, liquid chromatography, mass spectrometry, plasmaThe authors affirm that they have no conflicts of interest.
SummaryBackgroundThe concurrent impact of repeated low‐level summer sunlight exposures on vitamin D production and cutaneous DNA damage, potentially leading to mutagenesis and skin cancer, is unknown.ObjectivesThis is an experimental study (i) to determine the dual impact of repeated low‐level sunlight exposures on vitamin D status and DNA damage/repair (via both skin and urinary biomarkers) in light‐skinned adults; and (ii) to compare outcomes following the same exposures in brown‐skinned adults.MethodsTen white (phototype II) and six South Asian volunteers (phototype V), aged 23–59 years, received 6 weeks’ simulated summer sunlight exposures (95% ultraviolet A/5% ultraviolet B, 1·3 standard erythemal doses three times weekly) wearing summer clothing exposing ~35% body surface area. Assessments made were circulating 25‐hydroxyvitamin D [25(OH)D], immunohistochemistry for cyclobutane pyrimidine dimer (CPD)‐positive nuclei and urinary biomarkers of direct and oxidative (8‐oxo‐deoxyguanosine) DNA damage.ResultsSerum 25(OH)D rose from mean 36·5 ± 13·0 to 54·3 ± 10·5 nmol L−1 (14·6 ± 5·2 to 21·7 ± 4·2 ng mL−1) in phototype II vs. 17·2 ± 6·3 to 25·5 ± 9·5 nmol L−1 (6·9 ± 2·5 to 10·2 ± 3·8 ng mL−1) in phototype V (P < 0·05). Phototype II skin showed CPD‐positive nuclei immediately postcourse, mean 44% (range 27–84) cleared after 24 h, contrasting with minimal DNA damage and full clearance in phototype V (P < 0·001). The findings did not differ from those following single ultraviolet radiation (UVR) exposure. Urinary CPDs remained below the detection threshold in both groups; 8‐oxo‐deoxyguanosine was higher in phototype II than V (P = 0·002), but was unaffected by UVR.ConclusionsLow‐dose summer sunlight exposures confer vitamin D sufficiency in light‐skinned people concurrently with low‐level, nonaccumulating DNA damage. The same exposures produce minimal DNA damage but less vitamin D in brown‐skinned people. This informs tailoring of sun‐exposure policies.
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