The aim of this study was to identify and characterize aetiological and predisposing factors in denture-related stomatitis (DRS), by means of a case-control, transversal study, in a large cohort of 140 persons wearing removable maxillary polymethylamethacrylate prostheses. Data were obtained by (1) a questionnaire that included the identification of the subject, demographic and social data, medical history and behaviour; (2) intra-oral examination; (3) evaluation of the prosthesis; (4) microbiological examination; (5) yeast identification and analyses using Epi-info and the chi-square test. Results showed significant associations between DRS and yeasts, gender, age and alcohol consumption. We also found a significant relationship between the presence of yeasts and hyposalivation and decreased salivary pH. We found a highly significant difference between groups with and without DRS concerning the presence or absence of yeasts, regardless of the sample origin. Most cases of DRS showed the presence of Candida albicans. The results confirm a highly significant difference between groups with and without DRS concerning the presence or absence of yeasts.

The results reinforced the idea that C. albicans is the most frequent and can be the most pathogenic yeast in oral candidosis. However, the strains isolated from DRS patients and healthy individuals showed the same virulence factors. It seems that several virulence attributes are involved in the infective process but no single factor contributes to Candida virulence. Candida dubliniensis was absent in the oral cavity of individuals with and without DRS.

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Denture-related stomatitis (DRS) is the most common condition affecting removable-denture wearers, and Candida albicans the most frequent pathogenic agent. Systemic antifungal treatment is indicated but recurrences are frequent. The aim of this study was to characterize the oral load, fluconazole susceptibility profile and genotypic variability of oral C. albicans isolates from patients with DRS before (T0), immediately after fluconazole treatment (Tat) and after 6-months follow-up (T6m). Eighteen patients presenting DRS and treated with fluconazole were followed at the Faculty of Dentistry of Oporto University. Seventy C. albicans isolates were obtained and identified using standard cultural and biochemical multi-testing. Fluconazole susceptibility was tested by E-test®. Microsatellite-primed PCR was performed to assess the genotypic variability of C. albicans isolates. The patients’ mean age was 58.0±3.2 years, and 55.6%/44.4% had total/partial dentures. Before treatment, 22.2%, 44.4% and 33.3% of the patients presented DRS type I, II or III, respectively. Fluconazole treatment healed or improved DRS in 77.8% of the patients, accompanied by an 83.5% reduction in oral C. albicans load. However, after 6-months, oral C. albicans load increased significantly and DRS severity was similar to the one observed before treatment. Moreover, the prevalence of patients presenting fluconazole resistant isolates of C. albicans increased significantly throughout the study: T0-5.6%, Tat-10.0% and T6m-42.9%. A change in the genotypic variability of C. albicans isolates was also verified, being mostly associated to fluconazole susceptibility profile change. In conclusion, fluconazole presents a good short-term DRS treatment efficiency, but may be associated to a long-term emergence of C. albicans fluconazole resistance.

The inter-rater reliability of the Brazilian version of the Recent Life Events Interview, developed by Paykel in order to evaluate stressful life events, was studied in 21 psychiatric inpatients and 20 psychiatric outpatients. Data concerning the agreement between the two raters showed a satisfactory reliability for specific occurrence of events, month of occurrence, independence of events and negative objective impact. This preliminary study suggests that the Brazilian version of the Recent Life Events Interview is a reliable instrument for the assessment of life events in Brazilian samples.

Atualmente, um dos instrumentos mais utilizados em estudos envolvendo eventos de vida tem sido a entrevista para eventos de vida recentes desenvolvida por Eugene Paykel (UK). Trata-se de uma entrevista semi-estruturada composta por 64 itens divididos em dez categorias: trabalho, educação, finanças, saúde, luto, migração, moradia, problemas legais, família e relacionamentos sociais. A entrevista de Paykel já foi traduzida para o francês, o italiano, o alemão, o bengali, o Kannada (um dialeto indiano) e o arábico. O objetivo do presente trabalho foi tornar o aludido instrumento disponível para pesquisas sobre eventos de vida nos países de língua portuguesa, mediante sua tradução validada. A entrevista de Paykel foi traduzida para o português por um tradutor profissional e por uma psiquiatra brasileira bilíngüe. Em seguida, a versão em português foi retrotraduzida para o inglês por um psiquiatra inglês bilíngüe e enviada para o Professor Paykel que emitiu parecer favorável quanto à validade da tradução.

O objetivo deste trabalho foi avaliar a qualidade fisiológica de sementes da sempre-viva (Syngonanthus elegans (Bong.) Ruhland) colhidas em diferentes épocas e locais e submetidas ao armazenamento. Capítulos de S. elegans foram colhidos em duas áreas de ocorrência natural no Campus II da Universidade Federal dos Vales do Jequitinhonha e Mucuri - UFVJM, em Diamantina, MG, em um Neossolo Quartzarênico Órtico típico (local 1) e Neossolo Quartzarênico Hidromórfico típico (local 2), em cinco épocas (04/07/05, 19/07/05, 22/08/05, 21/09/05 e 20/10/05) e, posteriormente, submetidos a testes para avaliação do vigor e da germinação da semente (Experimento 1). Os capítulos colhidos em 04/07/05 (locais 1 e 2) foram acondicionados em sacos de polietileno transparente e armazenados no Laboratório de Sementes em temperatura ambiente (20º±2ºC) ou em geladeira (5º±2ºC), por zero, quatro, oito e 12 meses. Após esses períodos, as sementes foram novamente avaliadas quanto ao vigor e à germinação (Experimento 2). O delineamento experimental foi o inteiramente casualizado, no esquema fatorial 5x2 (época x local de colheita) para o Experimento 1; e o esquema fatorial 4x2x2 (armazenamento x local de colheita x temperatura de armazenamento) para o Experimento 2, com quatro repetições. A qualidade fisiológica da semente de S. elegans variou em função da época e do local de colheita. Durante o armazenamento, o vigor e a germinação não variaram com o local de colheita e a temperatura de armazenamento. O armazenamento por 12 meses em geladeira ou temperatura ambiente melhorou a qualidade fisiológica da semente.

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