The NRF2-ARE pathway
is an intrinsic mechanism of defense against
oxidative stress. Inhibition of the interaction between NRF2 and its
main negative regulator KEAP1 is an attractive strategy toward neuroprotective
agents. We report here the identification of nonacidic tetrahydroisoquinolines
(THIQs) that inhibit the KEAP1/NRF2 protein–protein interaction.
Peptide SAR at one residue is utilized as a tool to probe structural
changes within a specific pocket of the KEAP1 binding site. We used
structural information from peptide screening at the P2 pocket, noncovalent
small-molecules inhibitors, and the outcome from an explorative SAR
at position 5 of THIQs to identify a series of neutral THIQ analogs
that bind to KEAP1 in the low micromolar range. These analogs establish
new H-bond interactions at the P3 and P2 pockets allowing the replacement
of the carboxylic acid functionality by a neutral primary carboxamide.
X-ray crystallographic studies reveal the novel binding mode of these
molecules to KEAP1.
A novel approach for multivalent targeting by using gold nanoparticles noncovalently decorated by tight functionalization with a cone-glycocalixarene bearing four mannose units is reported. The targeting efficiency of these multivalent nanoparticles is shown to be remarkably improved compared to that of nanoparticles bearing a monovalent mannosylated derivative.
SummaryFour novel calix[4]arene-based glycoclusters were synthesized by conjugating the saccharide units to the macrocyclic scaffold using the CuAAC reaction and using long and hydrophilic ethylene glycol spacers. Initially, two galactosylcalix[4]arenes were prepared starting from saccharide units and calixarene cores which differ in the relative dispositions of the alkyne and azido groups. Once the most convenient synthetic pathway was selected, two further lactosylcalix[4]arenes were obtained, one in the cone, the other one in the 1,3-alternate structure. Preliminary studies of the interactions of these novel glycocalixarenes with galectin-3 were carried out by using a lectin-functionalized chip and surface plasmon resonance. These studies indicate a higher affinity of lactosyl- over galactosylcalixarenes. Furthermore, we confirmed that in case of this specific lectin binding the presentation of lactose units on a cone calixarene is highly preferred with respect to its isomeric form in the 1,3-alternate structure.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.