Background: As part of the Survey of Health, Ageing and Retirement in Europe (SHARE) study, dried blood spot (DBS) samples were obtained for measurement of potential biological biomarkers, among those vitamin D. Unfortunately, no studies describe the impact of high temperatures on DBS samples and vitamin D measurements. Materials and methods: Capillary samples were collected on DBS cards from 40 outpatients (median age 78 years) along with venous blood samples. To mimic the different environmental and temporal challenges during collection and shipment until final storage in the SHARE study, DBS cards were stored at different temperatures, at time span and with/without freeze-thaw. Vitamin D concentrations in venous plasma samples was measured by conventional immunoassay (on Architect i2000SR), while vitamin D concentrations in DBS samples were measured using LC-MS/MS with a well-described extraction method and with relevant calibration and comparison with a reference method. Results: Vitamin D measured in DBS samples did not differ significantly from venous plasma measurements under the different storage conditions tested. The optimal vitamin D correlation between the two matrices were by storage at either 21 °C or 35 °C for four days (r = 0.9060 and 0.9026, resp.). Freeze-thaw of the DBS samples did not have any significant effect. Conclusion: We find that vitamin D measured in DBS samples do not differ significantly from venous plasma measurements despite storage at different temperatures and freeze-thaw, which enables the use of DBS in multicenter studies taking place under alternating temperature conditions.
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