The aim of this study was to investigate the species diversity of microbiota isolated from soumbara, a traditional alkaline-fermented food and the microbial distribution in six major producing regions in Côte d'Ivoire. Ribosomal gene sequencing revealed that the Ivorian soumbara is associated with a wide microbial diversity essentially composed of 10 Bacillus species representing more than 90% of total microbial isolates, 3 species of lactic acid bacteria (2.48%), 2 species of Staphylococcus (1.42%), 8 fungal species (4.61%) and 1.06% of other microorganisms. However, the PCR-RFLP revealed a very weak intraspecific diversity as strains belonging to the same species showed a single restriction profile. Moreover, the microbiota associated with Ivorian soumbara consisted in a core and autochthonous microbiota namely B. subtilis and B. velezensis that were consistently present in samples from all regions studied and an incidental microbiota that was variable through the regions. Among the variable microorganisms, the presence of potential pathogen opportunistic such as Staphylococcus pasteuri, Staphylococcus sciuri and toxinogen species such as B. cytotoxicus and Aspergellus flavus were particularly found. The core microbiota is believed to be involved in the normal process of African locust beans into soumbara and can serve as potential starter whereas the incidental and variable microbiota rather undesirable may be involved in the spoilage or food-born disease. In this study, many species were described for the first time in soumbara, and revealed a new biodiversity of microbiota associated with soumbara in Côte d'Ivoire.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.