The X and Y chromosomes have been associated with malignancy in different types of human tumors. However, the function of sex chromosome aneuploidies (SCAs) is not yet fully known, it would be interesting to understand what the SCAs may be doing in sarcoma tissue.The aim of this study: It was to evaluate, the numerical aberrations of chromosomes X and Y in a patient with undifferentiated pleomorphic sarcoma (UPS), using fluorescence in situ hybridization (FISH) and by cytogenetic techniques. Materials and Methods:A patient with UPS underwent. The FISH technique was used to sarcoma tissue ;furthermore, standard technique for cultivation of lymphocytes from peripheral blood of the patient was used for identification of Y and X chromosomes. Results:A total 433 cancer cells were analyzed FISH. SCAs were found in 28.4%(123 cells) of 433 cancer tissue-cells (please explain -SCAs were found in 28.4% of all cells or numerically in 123?). In the blood, the SCAs were found in the 5.6% of 250 cells analyzed (how many cells were analyzed?). 72.4% and 27.6% of 123 cells with SCAs had the addition and loss of one or more extra X or Y chromosomes in cancer tissue, respectively. There was a significant difference in the frequencies of the increase (123 SCAs ) and loss of sex chromosomes, and the increase in sex chromosomes was higher in the tumor tissue (p<0.005). More specifically, polysomies were observed in 72.4% of the cells with SCAs. The other common karyotype was the loss of one Y (LOY), which was observed in 26.8% of the cells with SCAs. LOY were observed to be most frequent in blood of the patient (64.3%). Conclusion:Our results may suggest that the genetic instability associated with sex chromosome polysomies and LOY may account for the considerable potential for risk of sarcoma tumors.
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