Water salinity is a critical cause of the decrease in quality of fodder plants. The use of saline water as an alternative to fresh water requires the production of elite plant varieties that can tolerate excess amounts of sodium chloride. In this study, we evaluated six genotypes of barley (Hordeum vulgare L.) used as sprout fodder for their tolerance to saline water conditions. The six genotypes were tested for their germination vigor, α-amylase activity, root system architecture (RSA) phenotyping, relative water content (RWC), chlorophyll content (ChC), reactive oxygen species accumulation (ROS), and total antioxidant capacity. Increasing the salt concentration caused a significant decrease in the germination time, α-amylase activity, germination percentage, ChC, and RWC of all the genotypes, but significant differences in the RSA and ChC were detected. In addition, the plasticity of these characteristics at the seedling stage increased their potential to select varieties that could produce high amounts of green fodder when fresh water alternatives are used. Strong and positive correlations were detected between the green carpet formed under the salt treatment and ChC at the seedling stage for a local genotype and G134.
There is a demand for an increase in crop production because of the growing population, but water shortage hinders the expansion of wheat cultivation, one of the most important crops worldwide. Polyethylene glycol (PEG) was used to mimic drought stress due to its high osmotic potentials generated in plants subjected to it. This study aimed to determine the root system architecture (RSA) plasticity of eight bread wheat genotypes under osmotic stress in relation to the oxidative status and mitochondrial membrane potential of their root tips. Osmotic stress application resulted in differences in the RSA between the eight genotypes, where genotypes were divided into adapted genotypes that have non-significant decreased values in lateral roots number (LRN) and total root length (TRL), while non-adapted genotypes have a significant decrease in LRN, TRL, root volume (RV), and root surface area (SA). Accumulation of intracellular ROS formation in root tips and elongation zone was observed in the non-adapted genotypes due to PEG-induced oxidative stress. Mitochondrial membrane potential (âΚm) was measured for both stress and non-stress treatments in the eight genotypes as a biomarker for programmed cell death as a result of induced osmotic stress, in correlation with RSA traits. PEG treatment increased scavenging capacity of the genotypes from 1.4-fold in the sensitive genotype Gemmiza 7 to 14.3-fold in the adapted genotype Sakha 94. The adapted genotypes showed greater root trait values, âΚm plasticity correlated with high scavenging capacity, and less ROS accumulation in the root tissue, while the non-adapted genotypes showed little scavenging capacity in both treatments, accompanied by mitochondrial membrane permeability, suggesting mitochondrial dysfunction as a result of oxidative stress.
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