Background Cymbidium goeringii belongs to the Orchidaceae, which is one of the most abundant angiosperm families. Cymbidium goeringii consist with high economic value and characteristics include fragrance and multiple flower colors. Floral scent is one of the important strategies for ensuring fertilization. However, limited genetic data is available in this non-model plant, and little known about the molecular mechanism responsible for floral scent in this orchid. Transcriptome and expression profiling data are needed to identify genes and better understand the biological mechanisms of floral scents in this species. Present transcriptomic data provides basic information on the genes and enzymes related to and pathways involved in flower secondary metabolism in this plant. Results In this study, RNA sequencing analyses were performed to identify changes in gene expression and biological pathways related scent metabolism. Three cDNA libraries were obtained from three developmental floral stages: closed bud, half flowering stage and full flowering stage. Using Illumina technique 159,616,374 clean reads were obtained and were assembled into 85,868 final unigenes (average length 1194 nt), 33.85% of which were annotated in the NCBI non redundant protein database. Among this unigenes 36,082 were assigned to gene ontology and 23,164 were combined with COG groups. Total 33,417 unigenes were assigned in 127 pathways according to the Kyoto Encyclopedia of Genes and Genomes pathway database. According these transcriptomic data we identified number of candidates genes which differentially expressed in different developmental stages of flower related to fragrance biosynthesis. In q-RT-PCR most of the fragrance related genes highly expressed in half flowering stage. Conclusions RNA-seq and DEG data provided comprehensive gene expression information at the transcriptional level that could be facilitate the molecular mechanisms of floral biosynthesis pathways in three developmental phase’s flowers in Cymbidium goeringii, moreover providing useful information for further analysis on C . goeringii, and other plants of genus Cymbidium. Electronic supplementary material The online version of this article (10.1186/s12870-019-1940-6) contains supplementary material, which is available to authorized users.
The number of flower types filed and registered in the Korea Seed & Variety Service has increased each year. Of the 7,731 crops registered to date, flowers account for 53%, with 4,123 cases. This seems to reflect the nature of flowers with their various shapes and colors, as well as rapid change in varieties. The 1980s was seen as the birth of different flower varieties, with a focus on the collection of genetic resources for each flower type, the selection of chrysanthemums and rose varieties, and culturing of trumpet lilies and carnations. The 1990s was an era of globalization and the import of agricultural products, which enabled research on new flower varieties and the mass production of seedlings to develop strategies for export. In 1995, the Seed Industry Act was implemented and the cultivation of varieties actively began with Korea joining the International Union of the Production of New Varieties of Plants (UPOV); research on breeding and producing high quality seedlings for development of flower types such as lilies, carnations, chrysanthemums, roses, hibiscus, gerberas, gladioli, herbaceous flowers, cacti, orchids, and various other novel floral crops were expanded. In particular, support measures to promote private breeding were also devised. In the 2000s, breeding technology was stabilized, which led to the development of many new varieties at national and local levels, even by common citizens. Penetration rates of chrysanthemums, roses, and orchids, whose main focus was breeding, increased from 1% in 2000 to 5.8% in 2008 and 27.3% in the 2010s. Currently, cactus dominates the world market, with domestic varieties accounting for 70% of the world's trade, while freesias account for 60.4%, showing the high contribution of domestic small area crops. 'Baekma' (chrysanthemums), 'Shiny gold' (freesia), 'Deep purple' (rose), and 'Woori tower' (lily) are representative examples of domestic varieties bred in Korea. In the future, the development of varieties tailored to various consumers will be promoted, along with research on the production of virus-free seedlings, genome technology for enhancing breeding efficiency, and adding high value to the flower industry.
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