Locally known as top predator fishes, peacock bass or cichlids are invasive in Malaysia's freshwater ecosystem. Due to the nature of these fish, which involves hiding beneath the water's surface, detection probability for these species are often poor, especially when utilizing the traditional capture-survey method. As a result, measuring environmental DNA (eDNA) is a relatively new, noninvasive method that can be used to determine the range of these invasive fishes. Here, we outline the plan. For three selected invasive Cichla species C. kelberi, C. ocellaris, and C. monoculus it was necessary to develop small fragment (280-400 bp) specific-specific primers based on mitochondrial DNA (mtDNA) COI gene sequences. It was also necessary to determine how to extract high-quality DNA and e-DNA, as well as how to validate primers for specificity, (iv) developed and tested a new quantitative PCR (qPCR) assay to detect the presence of C. kelberi, and C. ocellaris, environmental DNA (eDNA) in water samples. The COI gene can be utilized to create species-specific primers for specific species, according to recent research. To improve the specificity of primers, a few primer-design factors must be changed. With recommendations for further references for the researcher, they were thoroughly examined. Furthermore, we discovered that e-DNA isolation must be completed within 22 days following capture in order to avoid producing false-negative results. Since just AGE (Agarose Gel Electrophoresis) analysis was required for the validation procedures, these data can be easily implemented in any fish genomics lab with a low-cost setting. Overall, eDNA analysis with speciesspecific primers represents a new monitoring tool in the detection of Aquatic Invasive Species (AIS) for management and conservation purpose.
Freshwater species are the most threatened group to be assessed to date by the IUCN. Freshwater fish has enormous importance as animal protein supplies for human, and it is estimated that 6% of the world annual animal protein supplies come from freshwater fishes. Barcoding projects have been initiated all over the world and the field is constantly growing. In Malaysia however, the field has not been deeply investigated and not many barcoding projects have been undertaken especially for freshwater fishes. The aim of our study is to support the progress of DNA barcode project, especially for inland reservoirs like Tasik Raban, Perak. A Standard methodology using Cytochrome c oxidase subunit 1 (COI) marker was developed to ensure native fishes are barcoded taxonomically and molecularly and ready to be accessed through online databases. Such public references can help increase awareness on local fish diversity management. Information on taxonomy and molecular characterization can be used to plan further conservation programmed especially for depleted, unrecognized, and cryptic native species.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.