The effect of early posthatch local feed in pectoralis muscle of Jawa Super chicks (Gallus gallus domesticus) AIP Conference Proceedings 1755, 140003 (2016) Abstract. Recent study on antibiotic produced by endophytic bacteria become emerging to fight against the increasing of drug-resistant bacteria. Twenty eight isolates of endophytic bacteria from Purwoceng (Pimpinella pruatjan Molkend) have been screened for their potential in preventing growth of pathogens, including Staphylococcus aureus and Pseudomonas aeruginosa. In the present study four isolates (GP11, GP12, RP6 and DG1) and another three isolates (GP2, GP14 and DG1) showed the activity to prevent the growth of S. aureus and P. aeruginosa ATCC 27853, respectively. Bioactive compounds were extracted from culture broth using ethyl acetate, followed by Thin layer chromatography (TLC) for metabolic profiling to identify the chemical class of compounds. Taxonomic status of the isolates was determined by BLAST analysis based on 16S rRNA sequences. The result of inhibition zone from 500 mg · mL -1 crude extract DG1, GP11, GP12 and RP6 against S. aureus respectively are 0.78 cm, 1.18 cm, 0.95 cm and 1.2 cm, whereas for 25 mg · mL -1 crude extract DG1, GP2 and GP14 against P. aeruginosa respectively are 0.144 cm, 0.151 em and 0.095 cm. TLC assay showed that endophytic bacteria produce alkaloid compound and from the BLAST analysis based on 16S rRNA sequences isolates closely related to Bacillus subtilis (GP11, GP12, GP14 and DG1), Bacillus cereus (RP6) and Enterococcus faecalis (GP2).
Kajian tentang senyawa antibakteri yang dihasilkan oleh bakteri endofit mulai bermunculan untuk mengatasi meningkatnya bakteri resisten obat seperti Escherechia coli. Bakteri endofit (GP14, GP2 dan DG1) yang diisolasi dari purwoceng (Pimpinella pruatjan Molkend) telah diketahui memiliki potensin dalam mencegah pertumbuhan bakteri patogen. Pada penelitian ini isolat GP14, GP 2 dan DG 1 menunjukkan aktivitas untuk mencegah pertumbuhan E. coli. Senyawa bioaktif diekstraksi dari kultur cair menggunakan etil asetat, diikuti dengan uji Thin layer chromatography (TLC) sebagai uji profil metabolik untuk mengidentifikasi golongan senyawa kimia. Uji TLC menunjukkan bahwa isolat GP14, GP2 dan DG1 menghasilkan senyawa alkaloid. Uji aktivitas antibakteri menggunakan metode paper disc diffusion dengan media TSA. Konsentrasi yang digunakan adalah 25 mg/ml, 50 mg/ml, 100 mg/ml dan 200 mg/ml. Hasil penelitian menunjukkan bahwa ekstrak kasar hasil ekstraksi etil asetat memiliki aktivitas antibakteri terhadap E. coli. Zona hambat terbesar ditunjukkan dari ekstrak kasar isolat DG1 pada konsentrasi 200 mg/mL yaitu 2.135 mm.
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