B-mode and Doppler ultrasonography in ruminants, as a technique has allowed establishing new concepts on the reproductive physiology of females, through the study of follicular dynamics and morphometry of the corpus luteum, therefore, the objective was to characterize by means of B-mode and Doppler ultrasonography the corpus luteum in recipient llamas. Thirty-seven recipient llamas were used (27 for B-mode ultrasonography and 10 Doppler) that were synchronized by applying buserelin acetate 0.0096 mg, nine days later 0.048 mg of prostaglandin F2α analog was applied two days later, the second dose of buserelin acetate was applied at the same dose to guarantee ovulation and subsequent formation of the corpus luteum, the evaluation in mode B of the morphometry consisted of observing the echotexture, area, diameter and volume of the corpus luteum with the use of a SonoStar SS-8 ® ultrasonograph at 6.5 MHz frequency and 6 cm depth equipped with a transrectal linear transducer; using the same technique with a Draminski 4Vet ® Doppler equipment, the percentage of luteal area of vascularization (% AVL) was determined. The data were subjected to a descriptive analysis being 0.119±0.032 cm2 of area, 12.7±1.7 mm of diameter and 0.84±0.32 cm 3 of CL volume, to determine the relationship between metric measures the Pearson correlation was used observing a positive relationship between area/diameter of 0.7506, area/volume of 0.9289 and diameter/volume of 0.6602, observing a high positive correlation between area and volume, finally the % AVL characteristics was 34.97 for the CL of recipient llamas. In conclusion, the morphometric characteristics evaluated in mode B (area, diameter and volume) have a positive correlation and the % AVL could be applied as a tool in the efficient reproductive management for the selection of recipients in camelids, understanding that it is one of the first reports of these characteristics in llamas 2021. Journal of the Selva Andina Animal Science ® . Bolivia. All rights reserved.
The objective of this study was to determine the effect of glycerol (GL), dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) at temperatures of 5 °C and 16 °C of equilibrium on sperm quality, after cryopreservation. 6 males were used in the semen collection, motility parameters were evaluated with the CASA system, viability, acrosomal integrity and mitochondrial activity by flow cytometry in fresh and thawed semen. We collected 30 ejaculates of which 10 were discarded for not fulfilling (volume ≥ 1 ml and total motility ≥ 60 %), the 20 ejaculates were mesclo in a tube to form pool carrying out the assessment of the initial quality of fresh semen, obtaining a total motility (MT) 61,6 ± 3,4%, progressive motility (MP) 51,7 ± 3,7%, viability 78,1 ± 9.1%, mitochondrial activity (MA) 77,4 ± 7,7 and acrosomal integrity 88,9 ± 11,5%. After cryopreservation no equilibrium temperature effect was observed, independently of cryoprotectant in the parameters of seminal quality. Cryoprotective effect (P≤ 0.05) was observed, presenting superior values in the sperm conserved in GL with respect to the rest of cryoprotectants, with significant differences (P ≤ 0,05) in MT, MP, viability and AM and without significant differences in acrosomal integrity. The equilibrium temperature of 16 °C was effective for cryopreservation of alpaca sperm. In addition, the 5% GL preserved better the functional characteristics of sperm compared to 7% (DMF and DMSO).
The objective of this research was to evaluate the use of the intravaginal device (CIDR) and estradiol cypionate in synchronization protocols, on oestrus presentation, pregnancy and birth rate in Creole sheep under high altitude conditions. The study was carried out in a non-reproductive season, in the peruvian highlands, using 57 ewes. Four experimental groups were formed: group T1 (n=14) and T3 (n=14), CIDR progesterone device for 7 days and 12 days, respectively; group T2 (n=15) and T4 (n=14), CIDR progesterone device for 7 days and 12 days plus 1 mg of estradiol cypionate, 24 hours after removal of the device in both groups, respectively. Estrous presentation was observed from CIDR removal to 48 hours using vasectomized rams and IATF was performed with fresh semen 48 hours after device removal. The pregnancy rate was determined by ultrasonography at 46 and 90 days after FTAI and the birth rate was recorded. The chi-square test was used for statistical analysis. There was no difference (P>0.05) between groups, for estrous presentation, but there was difference (P<0.05) for pregnancy rate at 46 days between groups with: T1 (42.9%), T3 (38.5%), T4 (21.4%), with respect to T2 (0%) (The difference in results are shown in Table 2). There was a difference (P<0.05) for the pregnancy rate at 90 days: T1 (35.7%) and T3 (30.8%), with respect to T4 (7.1%) and T2 (0 %). The birth rate showed differences (P<0.05) for T1 (28.6%) and T3 (28.6%), with respect to T2 (0%) and T4 (0%). In conclusion, a high pregnancy rate was obtained with CIDR for 7 and 12 days, compared to the use of CIDR plus estradiol cypionate. However, no births were obtained with progesterone plus estradiol cypionate treatment.
B-mode and Doppler ultrasonography in ruminants, as a technique has allowed establishing new concepts on the reproductive physiology of females, through the study of follicular dynamics and morphometry of the corpus luteum, therefore, the objective was to characterize by means of B-mode and Doppler ultrasonography the corpus luteum in recipient llamas. Thirty-seven recipient llamas were used (27 for B-mode ultrasonography and 10 Doppler) that were synchronized by applying buserelin acetate 0.0096 mg, nine days later 0.048 mg of prostaglandin F2α analog was applied two days later, the second dose of buserelin acetate was applied at the same dose to guarantee ovulation and subsequent formation of the corpus luteum, the evaluation in mode B of the morphometry consisted of observing the echotexture, area, diameter and volume of the corpus luteum with the use of a SonoStar SS-8 ® ultrasonograph at 6.5 MHz frequency and 6 cm depth equipped with a transrectal linear transducer; using the same technique with a Draminski 4Vet ® Doppler equipment, the percentage of luteal area of vascularization (% AVL) was determined. The data were subjected to a descriptive analysis being 0.119±0.032 cm2 of area, 12.7±1.7 mm of diameter and 0.84±0.32 cm 3 of CL volume, to determine the relationship between metric measures the Pearson correlation was used observing a positive relationship between area/diameter of 0.7506, area/volume of 0.9289 and diameter/volume of 0.6602, observing a high positive correlation between area and volume, finally the % AVL characteristics was 34.97 for the CL of recipient llamas. In conclusion, the morphometric characteristics evaluated in mode B (area, diameter and volume) have a positive correlation and the % AVL could be applied as a tool in the efficient reproductive management for the selection of recipients in camelids, understanding that it is one of the first reports of these characteristics in llamas 2021. Journal of the Selva Andina Animal Science ® . Bolivia. All rights reserved.
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