Rabbit has potency to produce meat since it has high growth rate, litter size, and short calving interval. Rabbit can utilize low nutritional feed and wide various of forages as its feed. Recently, Indigofera Zollingeriana (IZ) has been widely used as a green protein source in livestock. The used of IZ in rabbit feed seemed to be able to reduce production cost. The study had objective to observe the effect of IZ supplementation in feed to rabbit performance. A number of 72 heads of 12 weeks old of New Zealand white rabbits were used in the study. Rabbits were distributed in three groups of IZ supplementation levels (R1: 0 %; R2: 20 %, and R3: 30%). The supplementation was applicated for five weeks. Data were analysed using one- way ANOVA. The results showed that IZ supplementation effected body weight gain (BWG), feed conversion ratio, and dry matter and energy digestibility (P<0.05). The R2 was the optimal IZ supplementation in the study. The average BWGs of rabbits were 833.38 ± 232 g, 688.50 ± 88 g, and 485.63 ± 130 g for R1, R2, and R3, respectively. In conclusion, the IZ supplementation up to 20 % could be applicated in rabbit feed to reduce cost
Free radicals are atoms or molecules that have one or more unpaired electrons and are highly reactive. Antioxidants can inhibit the excessive oxidation reaction. The leaves of binahong (Anredera scandens (L.) Moq.) are one part of plants which contain flavonoid compounds and tannins that have scientific activity as antioxidants.This study aims to determine the bioautographic profile and to test the antioxidant activity of the ethanol extract of the leaves of Anredera scandens (L.) Moq. useing 2.2-diphenyl-1-pikrilhidrazil radical capture method. The extraction process produced 19.22% yield, 14.68% (14.684 grams) chloroform fraction, 11.22% (11.224 grams) n-hexane fraction, 3.09% (3.094 grams) ethyl acetate fraction, and 23.90% (23.904 grams) n-butanol fraction. The results of bioautographic profile test using KLT densitometry method proved that ethanol extract, chloroform fraction and ethyl acetate fraction have DPPH radical capture activities. The determination of total flavonoid content found that ethyl acetate fraction contained higher total flavonoids than other fractions. In the ethyl acetate fraction, high total flavonoid level and low IC50 values were obtained, while the n-hexane fraction had low flavonoid level and high IC50 values. Keywords: antioxidants, binahong leaves, free radicals, flavonoids, IC50.
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