Developing stimulus-responsive biomaterials with easy-to-tailor properties is a highly desired goal of the tissue engineering community. A novel type of electroactive biomaterial, the conductive polymer, promises to become one such material. Conductive polymers are already used in fuel cells, computer displays and microsurgical tools, and are now finding applications in the field of biomaterials. These versatile polymers can be synthesised alone, as hydrogels, combined into composites or electrospun into microfibres. They can be created to be biocompatible and biodegradable. Their physical properties can easily be optimized for a specific application through binding biologically important molecules into the polymer using one of the many available methods for their functionalization. Their conductive nature allows cells or tissue cultured upon them to be stimulated, the polymers' own physical properties to be influenced post-synthesis and the drugs bound in them released, through the application of an electrical signal. It is thus little wonder that these polymers are becoming very important materials for biosensors, neural implants, drug delivery devices and tissue engineering scaffolds. Focusing mainly on polypyrrole, polyaniline and poly(3,4-ethylenedioxythiophene), we review conductive polymers from the perspective of tissue engineering. The basic properties of conductive polymers, their chemical and electrochemical synthesis, the phenomena underlying their conductivity and the ways to tailor their properties (functionalization, composites, etc.) are discussed.

New advances in tissue engineering are being made through the application of different types of electrical stimuli to influence cell proliferation and differentiation. Developments made in the last decade have allowed us to improve the structure and functionality of tissue-engineered products through the use of growth factors, hormones, drugs, physical stimuli, bioreactor use, and two-dimensional (2-D) and three-dimensional (3-D) artificial extracellular matrices (with various material properties and topography). Another potential type of stimulus is electricity, which is important in the physiology and development of the majority of all human tissues. Despite its great potential, its role in tissue regeneration and its ability to influence cell migration, orientation, proliferation, and differentiation has rarely been considered in tissue engineering. This review highlights the importance of endogenous electrical stimulation, gathering the current knowledge on its natural occurrence and role in vivo, discussing the novel methods of delivering this stimulus and examining its cellular and tissue level effects, while evaluating how the technique could benefit the tissue engineering discipline in the future.

Abstract. This paper investigates the patterns and controls of aquifer-river exchange in a fast-flowing lowland river by the conjunctive use of streambed temperature anomalies identified with Fibre-optic Distributed Temperature Sensing (FO-DTS) and observations of vertical hydraulic gradients (VHG).FO-DTS temperature traces along this lowland river reach reveal discrete patterns with "cold spots" indicating groundwater up-welling. In contrast to previous studies using FO-DTS for investigation of groundwater-surface water exchange, the fibre-optic cable in this study was buried in the streambed sediments, ensuring clear signals despite fast flow and high discharges. During the observed summer baseflow period, streambed temperatures in groundwater up-welling locations were found to be up to 1.5 • C lower than ambient streambed temperatures. Due to the high river flows, the cold spots were sharp and distinctly localized without measurable impact on down-stream surface water temperature.VHG patterns along the stream reach were highly variable in space, revealing strong differences even at small scales. VHG patterns alone are indicators of both, structural heterogeneity of the stream bed as well as of the spatial heterogeneity of the groundwater-surface water exchange fluxes and are thus not conclusive in their interpretation. However, in combination with the high spatial resolution FO-DTS data we were able to separate these two influences and clearly identify locations of enhanced exchange, while also obtaining information on the complex small-scale streambed transmissivity patterns responsible for the very discrete exchange patterns. The validation of the combined VHG and FO-DTS approach provides an effective strategy for analysing drivers and controls of groundwater-surface water exchange, with implications for the quantification of biogeochemical cycling and contaminant transport at aquifer-river interfaces.

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