The male of Latonigena auricomis Simon, 1893 is described for the first time and the female is redescribed. New records are provided for Argentina, Brazil and Uruguay. Notes on the natural history and a potential distribution model of the species are presented in the Neotropical Region.

The taxonomy of the genus Latonigena Simon is updated after the study of the collection of the Museo Argentino de Ciencias Naturales (Buenos Aires). Two new species are described: L. pampa, n. sp., from Buenos Aires Province, Argentina, and L. pittieri, n. sp., from Aragua, Venezuela (the first record of a member of this genus from that country). Three species previously known from Brazil (L. turvo, L. lami, and L. santana) are newly reported from Argentina, and the female of the latter is here described for the first time. Zelotes zonatus (Holmberg) is considered nomen dubium.

A new genus of gnaphosid spiders, Almafuerte, gen. nov., is described for seven species from Southern South America. The new genus is diagnosed by a conspicuous retrolateral tegular projection on the male palp, and by having three teeth on the cheliceral retromargin. It comprises six species from Argentina, A. peripampasica sp. nov. (type species), from Central Argentina, also present in Uruguay, A. kuru sp. nov., from Santiago del Estero, A. vigorosa sp. nov., from Corrientes, A. goloboffi sp. nov., from Salta and Jujuy, A. remota sp. nov., from Santiago del Estero and Mendoza, and A. giaii (Gerschman & Schiapelli) comb. nov., here transferred from Echemus, from Santa Fe (the female is here described by the first time); and one species from Bolivia, A. facon sp. nov., from Cochabamba. Hypotheses of relationships of the new taxa are briefly discussed, in the context of the previously proposed groupings: the subfamilies Echeminae and Drassodinae (sensu Platnick 1990) and the "Echemus group" (sensu Murphy 2007).