Anethum graveolens L. is a plant belong to umbelliferae family. The aim of this study compare the phytochemical of different volatile oil extract from fresh air plant and commercial dry A. graveolens from market in Karbala Iraq, by soxhlet apparatus for a bout 24 hours, The results indicated the percentage of volatile oils differed significantly as a result of being affected by the type of plant, The fresh A.graveolens gave the height percentage of the volatile oils extract (4. 17%, while the dry A. graveolens gave the lower percentage of the volatile oils extract (2. 5%), Then phytochemical screen wer performed for fresh A. graveolens extract and revealed positive results for Saponin, Phenol and Tanin, Glycoside, Alkaloid and Flavonoid while the dry showed positive results for Saponin, Phenol and Tanin while Glycoside, Alkaloid and Flavonoid negative results. The Gas chromatography mass-spectrometer analysis were done for the fresh results showed that essential oil of fresh A.graveolens 30 compounds and had the highest compound concentration Hexadecanoic acid, methyl ester reaching it is percentage (28.93%) followed 9-Octadecenoic acid, methyl ester (27.60%), Then 9.12-Octadecadienoic acid compound concentration (10.52%). concentration the dry A.graveolens, the study showed that it contain 30 compound and that the compound Cyclohexanecarboxylic acid, pentad has the highest compound concentration of (21.41%) followed by the Thymol 12.41%, then 2-Dodecylcycobutanone with concentration of (8.81%).

Petroselinum sativum L : is plant belong to     Umbellifera Family , The aim of this study identification phytochemical compounds by using Gas Chromatography Mass- Spectrometer and Percentage of volatile oils extracted and determined the effect oils extract on four of bacteria ( Escherichia Coli , Pseudomonas putida and Enterococcus faecales ) . P. sativum L. fresh from Karbalaa city. Iraq in 1/2/ 2022 during the flowering and volatile oils extract by soxhlet apparatus for about 20 hours , The results indicated the percentage of volatile oils  for the  P. sativum L. gave the height  percentage of the volatile oils extract (5%), The Gc/mass analysis of the compounds in the extraction of  P. sativum L. shown that essential oil 25 compounds and had the highest compound concentration Hexadecyn-1-ol reaching it is percentage (25.73%), followed -Isopropyl-2-methylphenyl carbanlate (18.45%) , Then 9-Octadecenoic acid, (E)- compound concentration (17.89%) . The antibacterial effected of oils depending were concentration increasing my due be the high concentration was supreme effective .

This study emphasized on the identification and detection the secondary metabolism compounds different of volatile oils by Gas Chromatography Technique of Ocimum basilicum L. and Mentha spicata L plant from Labiate (Lamiaceae) family cultivated in Iraq by suxhlet apparatus. the medicinal importment acquired this study and to know the active compound in the volatile oil was identified in plant extract of aerial parts by Gas Chromatography Mass, The result showed that of (80g) of O.basilicum and M. spicata has produced volatile oil ( 2.5% and 3. 75%) respectively, The study has shown that essential oil of O.basilicum 25 compounds and had the highest compound concentration Estragole reaching it is percentage (23.46%) followed Cyclohexanol,5-methyl-2-(1methyl ethyl) (14.19%), Then Tetrapentaconate, 1,54-dibromo compound concentration (8.99%). concentration the M. spicata, the study showed that it contain 25 compound and that the compound Isopropyl linoleate has the highest compound concentration of (21.83%) followed by the Cyclohexanol,5-methyl-2-(1-methyl ethyl) 13.38%, then Octadecenoic acid (E) with concentration of ( 7.75%). The aim of this study compare the phytochemical of different volatile oil extract of O. basilicum L. and M. spicata L.

Background: This search was carried to investigate the phytochemical of Petroselinum sativum L.parsley and the antioxidant effect of parsley volatile oils leaves extract on oxidative stress [MDA (Malondialdehyde enzyme), LPO ( lipid peroxidation enzyme ) level and antioxidant glutathione enzyme (GSH)] in adult male albino rats.Objective: This research work aimed to study the antioxidant effect of volatile oils of parsley leaves extract against environment-induced free radicals oxidative stress in male albino rats. Methods: 30 male adult rats were used. Rats were divided into five Groups: G1 (Control group) received only 0.9% normal saline, G2 received P.sativum L volatile oils extracts 50 Mg / Kg B.W, G3-received P.sativum L volatile oils of extracts 100 mg/kg B.W, G4-received P.sativum L volatile oils of extracts 200 mg/kg B.W, G5-received P.sativum L volatile oils extracts 250 mg/kg B.W volatile oils of extracts. The extract was injected (i.p.) in some rats for 20 days and 30 days and 60 days. Then blood samples were collected for measuring MDA, LPO and GSH levels in experimental groups and compered with control. Results: Results indicated that MDA and LPO levels were significantly decreased at concentrations of 50, 100, 200 and 250 mg/kg B.W., while GSH levels significantly increased at the same concentrations compared to the control group. Conclusions: There was a dose-dependent marked decrease in the levels of MDA and LPO and a dose-dependent marked increase glutathione in male rats treated with parsley extract.