In this study the leaves and fruits of wild raspberry (Rubus idaeus L.) populations from the central Balkan region were examined to determine the level of secondary metabolites and related antioxidant activity, as well as biological activity, upon existing ethnobotanical evidence, primarily linked to gastrointestinal disorders. The values obtained for total phenols ranged from 59.68 to 96.83 mg GA g-1 and 24.29 to 38.71 mg GA g-1 in leaf and fruit extracts, respectively. The highest values of tannins and anthocyanins were determined for leaf extracts from a population of east Serbia at a level of 1.27 mg mL-1 and 9.00 mg mL-1. Antioxidant activity was evaluated by measuring the scavenging capacity of the extracts on DPPH. Higher antioxidant activity was detected in the leaf extracts than in the fruit extracts. Leaf and fruit extract were the most effective against Escherichia coli (ATCC 8739). Anticancer activity was studied on a human colorectal cancer cell line HCT-116. Leaf extracts exhibited anticancer activity with IC50/24 h 162.38 μg mL-1 and IC50/48 h 95.69 μg mL-1. Wild raspberry leaf and fruit extracts contain numerous secondary metabolites providing marked antioxidant, antimicrobial and anticancer activity.
Variations in abiotic environmental factors have significant effects on quantity and quality of secondary metabolites, which is particularly important for plant species that possess biologically active compounds. The purpose of this study is determination of the total phenolic content, flavonoid concentration, and antioxidant activity of the different parts of Inula helenium L. (Asteraceae) sampled from different populations and in different time periods. The amounts obtained for the total phenolics varied from 16.73 to 89.85 mg of gallic acid (GA)/g. The concentration of flavonoids ranged from 9.32 to 376.22 mg of rutin (Ru)/g. The IC50 values of antioxidant activity determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical method varied from 161.60 to 1563.02 μg/ml. The inflorescence and roots possessed high concentration of phenolic compounds and significant antioxidant activity, while leaves contained the highest concentration of flavonoids. Additionally, the quantity of the phenolics, as well as antioxidant activity, significantly varied among the different populations due to different impacts of environmental factors. This research showed that I. helenium represents an abundant source of bioactive substances, and that the quantity of these compounds greatly differs among the different populations as well as in the same populations regarding the different time periods as well as plant parts.
The purpose of this comparative analysis is the determination of the total quantity of metals (Mg, Ca, K, Ni, Fe, Mn, Zn, Cu, Cr and Pb) in soil samples, above ground plant parts and tea made of plants Teucrium montanum and T. chamaedrys from different serpentine and calcareous habitats as well as of the total quantity of phenolic compounds and antioxidant activity. The obtained results showed that the quantities of certain metals (Mg, Fe, Ni and Mn) in the soil from the serpentine habitats were greater in comparison with other metals (Ca, Zn and Pb) which were more frequently found in the soil from the calcareous habitats. The results demonstrated that the analysed plant samples from the serpentine habitats contained higher quantity of Fe, Ni and Cr as opposed to the plant samples from the calcareous habitats which contained greater quantity of Ca and Zn. Although the studied species accumulate analysed metals in different quantities, depending on the substrate type, they are not hyperaccumulators of these metals. The use of these species from serpentine habitats for tea preparation is safe to a great extent, because in spite of the determined metal absorption by plant organs, the tea does not contain dangerous quantity of heavy metals. The results showed greater total quantity of phenolic compounds and the higher level of antioxidant activity in the plant samples from serpentine habitats in comparison with the samples from calcareous habitats, which is an indicator of one of the mechanisms of adaptation to the serpentine habitat conditions.
Many compounds from the phenolic group, flavonoids in particular, are well-known antioxidants, although their role in plant response to stress is debatable. The aim of this study was to determine the variability of the phenolic content and the antioxidant activity of Olea europaea leaf samples from different habitats. The determination included measurement of the total quantity of phenolics, the flavonoid content, as well as the antioxidant activity of the two types of methanolic leaf extracts of O. europaea from several natural habitats in the Mediterranean region (Tunisia, Malta and Montenegro) and from cultivated conditions (France and Serbia). The results showed that both the total quantity of phenols and flavonoids as well as the intensity of antioxidant activity in the two types of extracts largely depended on the type of habitat. The total quantity of phenols and flavonoids was greater in the samples from cultivated plants which demonstrated the significance of certain conditions in terms of the correlation between the intensity of primary and secondary metabolism. However, the values of antioxidant activity in both types of extract were higher in the samples from natural habitats. The results showed that plants from natural habitats contain secondary metabolites with high biological activity. It could be speculated that these active substances play an important role in the adaptation of plants to the stress caused by arid conditions.
ABSTRACT. This paper presents the results of a screening of nine different extracts from the plant parts (leaves, flowers and stems) of Euphorbia cyparissias for total phenolic content, concentration of flavonoids and in vitro antioxidant activity. Мain reason for this study is the determination of these parameters and their variability among plant parts and plant extracts obtained by different solvents, respectively. Obtained amounts for total phenolic content ranged from 10.76 to 40.72 mg GA/g. The concentration of flavonoids varied from 34.32 to 134.34 mg Ru/g. The IC 50 values of antioxidant activity varied from 88.48 to 2891.08 µg/ml. Results obtained from the different plant parts were of uneven value. Great variability of the studied parameters was observed when comparing the effectiveness of the used solvents. The acetone extracts from stems contain the greatest concentrations of phenolic compounds, especially flavonoids, and showed high antioxidant activity. According to our research, plant parts from E. cyparissias can be regarded as promising candidates for natural plant sources with high value of biological compounds.
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