Purification of begomovirus from infected ash gourd leaf samples using anti-ACMV antibody-conjugated magnetic nanoparticles (Ab-MNPs) and their characterization.
A simple method for the rapid synthesis of selenium nanoparticles (SeNPs) by the reduction of selenium dioxide using an aqueous egg white lysozyme solution (stabilizing agent) and ascorbic acid solution (reducing agent) has been described. The formation of SeNPs is assured by characterization with UV-Vis, FT-IR, XRD and morphological characters that were observed using TEM analyses. The absorbance of the SeNPs is found at 250 nm. The TEM images show that the SeNPs are uniform and in spherical shape of size 40-60 nm. The crystalline nature of SeNPs is assured by XRD analysis. The antibacterial property of synthesized SeNPs was tested against the pathogenic bacteria, i.e., Bacillus subtilis, Bacillus cereus, Escherichia coli, Staphylococcus pneumoniae, Proteus mirabilis and Klebsiella pneumoniae. SeNPs showed more antibacterial activity against B. subtilis (19 mm) and Streptococcus pneumoniae (15 mm) as compared with commercially available antibiotics such as streptomycin and erythromycin. The SeNPs synthesized via this process has proficient antimicrobial activity against pathogenic bacteria. SeNPs could play a significant role in pharmaceutical industries for the development of efficient antibiotic agents.
Colletotrichum gloeosporioides is the most destructive endophytic plant-pathogenic fungi causing Anthracnose disease in a wide number of economically important plants throughout the world. By far there is no existing methods possessing an effective disease management over the outbreak of anthracnose disease and it's precede to huge economic losses for formers and plant tissue culture laboratories. In order to find a new and effective control measure over these endophytes, with aid of nanotechnology, we synthesized bioactive bile salt of sodium deoxycholate (NaDC) capped silver nanoparticles and satisfactorily characterized. Further it was employed to control the endophytic fungus through in vitro direct and indirect model system with time dependant/light mediated incubation manner. In our findings we achieved fivefold synergistic effect of NaDC-capped AgNPs with their bioactive capping agent against Colletotrichum gloeosporioides upon analyzing different parameters with typical instruments. Moreover, it was evident that NaDC-capped AgNPs did not cause any phytotoxicity to treated plants revealed by molecular markers. Figure: 6. Direct antifungal activity: FESEM images of Colletotrichum gloeosporioides incubated with NaDC capped AgNPs at various timings shows degrees of cell damage: (a) 0 min (b) 30 min (c) 60 min (d) 90 min (e) 120 min and (f) 150 min.
A new era has begun in which pathogens have become useful scaffolds for nanotechnology applications. In this research/study, an attempt has been made to generate an empty cargo-like architecture from a high-profile plant pathogen of Squash leaf curl China virus (SLCCNV). In this approach, SLCCNV coat protein monomers are obtained efficiently by using a yeast Pichia pastoris expression system. Further, dialysis of purified SLCCNV-CP monomers against various pH strengthenened (5-10) disassembly and assembly buffers produced a self-assembled "Nanocargo"-like architecture, which also exhibited an ability to encapsulate the magnetic nanoparticles at in vitro. Bioinformatics tools were also utilized to predict the possible selfassembly kinetics and bioconjugation sites as well. The biocompatibility of "SLCNNV-CP-Nanocargo" particles was also evaluated by in vitro cancer cells, which eventually proved the particles to be versatile material for the next generation "nanotool" capable of housing various therapeutic or imaging agents..
Understanding the cellular and molecular events and identifying the appropriate treatment strategies for different foot ulcer grades will reduce recurrence of foot ulcer and lower limb amputation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.