Objectives
A bio-guided selection and identification of the most active compounds obtained from Algerian Pistacia atlantica desf. Subsp. atlantica.
Methods
An antioxidant activity guided fractionation was performed on buds’ extract using extensive chromatographic and spectroscopic techniques. The antioxidant potentials of isolated compounds and other unpurified fractions were evaluated against DPPH radicals using TLC plates and test tubes.
Results
The results showed that all isolated compounds and fractions exhibited eminent DPPH scavenging potential. Two coumarins (7-ethoxycoumarin and 7-hydroxy-5-methoxycoumarin) and two flavonoids (3′,5,7-Trihydroxy-4′-methoxyflavanone and 5,6,7,4′-tetrahydroxyflavonol-3-O-rutinoside) were isolated for the first times from the titled subspecies.
Conclusions
These results confirm that the species of P. atlantica is far from being exhausted of active compounds, especially polyphenols.
In the present paper, for the first time, we are interested to separate and identify some bioactive fractions isolated from the roots of a Saharan plant Anabasis articulata, which is widely used in traditional medicine against cancer. The crude methanolic extract of the roots was fractionated on column chromatography, and eluted with dichloromethane/methanol each time with increasing polarity of methanol; 17 fractions were separated. One of these fractions named F12 showed more antioxidant activity to scavenge DPPH free radical with percentage inhibition of 95.29%. F12 was separated by thin-layer chromatography (TLC) to give 12 compounds. A second preparative TLC of compound 2, which has antioxidative activity of 74.92%, provided the three phenolic acids M1, M2 and M3, analysed by high-performance liquid chromatography and UV-visible spectrophotometry.
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