Zebrafish are an important animal model, whose structure and function information can be used to study development, pathologic changes and genetic mutations. However, limited by the penetration depth, the available optical methods are difficult to image the whole-body zebrafish in juvenile and adult stages. Based on a home-made high-resolution polarization-sensitive optical coherence tomography (PS-OCT) system, we finished in vivo volumetric imaging for zebrafish, and various muscles can be clearly discerned by scanning from dorsal, ventral, and lateral directions. Besides structure information, polarization properties extracted from PS-OCT images provide abundant function information to distinguish different muscles. Furthermore, we found local retardation and local optic axis of zebrafish muscle are related to their composition and fiber orientation. We think high-resolution PS-OCT will be a promising tool in studying myopathy models of zebrafish.
Zebrafish brain imaging is very important for the study of brain disease and regeneration. We scanned the adult zebrafish brain before and after skull removal and monitored the recovery process of a head wound by polarization‐sensitive optical coherence tomography (PS‐OCT) in this paper. We analyzed the structure and polarization characteristics of the brain and skull in PS‐OCT images, and found their internal microstructure can be clearly identified with the polarization information. Further, we estimated the pigment distribution of the skull area and found that the density of pigment in skull is a critical factor of affecting zebrafish brain in vivo polarization imaging. Our results demonstrated that more features of brain can be displayed by introducing the polarization information, and proved high‐resolution PS‐OCT will play a great potential role in studying the zebrafish brain and skull.
Zebrafish is an important animal model, which is used to study development, pathology, and genetic research. The zebrafish skin model is widely used in cutaneous research, and angiogenesis is critical for cutaneous wound healing. However, limited by the penetration depth, the available optical methods are difficult to describe the internal skin structure and the connection of blood vessels between the skin and subcutaneous tissue. By a homemade high-resolution polarization-sensitive optical coherence tomography (PS-OCT) system, we imaged the polarization contrast of zebrafish skin and the zebrafish skin vasculature with optical coherence tomography angiography (OCTA). Based on these OCT images, the spatial distribution of the zebrafish skin vasculature was described. Furthermore, we monitored the healing process of zebrafish cutaneous wounds. We think the high-resolution PS-OCT system will be a promising tool in studying cutaneous models of zebrafish.
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