Ethanol extract of Pisonia aculeata (EPA) was evaluated for hepatoprotective and antioxidant activities in rats. The plant extract (250 and 500 mg/kg, p.o.) showed a remarkable hepatoprotective and antioxidant activity against carbon tetrachloride (CCl 4) -induced hepatotoxicity as judged from the serum marker enzymes and antioxidant levels in were compared with respective control. Results indicate the hepatoprotective and antioxidant properties of P. aculeata against CCl 4 -induced hepatotoxicity in rats.