Materials and methods2.1. Plant materials and RNA isolation 2-year-old Olea europaea L. europaea cv. Gemlik seedlings were obtained from Ataturk Horticultural Central Research Institute in Yalova.Total RNA was extracted from olive leaves using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Genomic DNA and chemical
Research ArticleThis work is licensed under a Creative Commons Attribution 4.0 International License.
One of the most prevalent environmental stresses that affects plants physically and physiologically is
the low temperature. Since low temperature adversely affects plant productivity Researchers
investigated the molecular processes that regulate the effects of cold stress on plants and discovered
many cold responsive genes as well as the control mechanisms that regulate them. CBF/DREB (C repeat
binding factor/dehydration response element binding factor) transcription factors induces the
expression of corresponding genes in plants in response to abiotic stress conditions such as cold,
drought and salinity. These transcription factors contribute to plant resistance to stress by activating
genes in various pathways. Genes encoding CBF transcription factors were first discovered in
Arabidopsis thaliana (L.). Later, Gene homologs were isolated and cloned from a variety of plants. They
belong to the AP2/EREBP protein family. In the present study, the full-length cDNA encoding homologue
of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long
cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1
sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using
Olea europea L. cv. Gemlik CBF1 sequence.
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