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Introduction: ESKAPE pathogens include Enterococcus faecium, Staphylococcus aureus, Klebsiella pnuemonia, Acienetobacter baumanii, Pseudomonas aeruginosa and Enterobacter species. Currently all these organisms are the main cause of hospital infections globally and they have the property to effectively escape the effect of antibacterial drugs. Unstoppable success of these superbugs will lead to unwinnable war. The success of these pathogens is mainly because of the mutations, modifications of LPS. As the crisis for the antibiotic resistance continues to grow, the latest IDSA (infectious disease society of America) "Bad Bugs, No Drugs" reports the urge for new antibiotics in the research and development pipeline and proposes steps to tackle the shortage. Objective: The aim of the study was to characterize the antimicrobial resistance in ESKAPE pathogens isolated from 330 culture positive clinical sample. Method: Antibiotic resistance was determined by VITEK 2 and manual method was done on Kirby baurer method. MIC was determined by VITEK 2 and E-Test according to CLSI guidelines. Result: Out of the total cases 63 percent of the culture has ESKAPE pathogens. Except for S. aureus multidrug resistance index of ESKAPE pathogens revealed on increasing trend. Conclusion: ESKAPE pathogens are commonly identified in alarming frequency and knowledge of antimicrobial resistance will be aided for empirical treatment.

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Background: Pseudomonas aeruginosa is an opportunistic gram-negative pathogen known for its ingenious mode of infection. The management of infections with Pseudomonas has been quite a challenge. The bacteria have intrinsic resistance against most of the routine antibiotics. Such a scenario places the health care delivery system at a challenging end point with very minimal options of care and increased rates of morbidity and mortality. This study was done to assess the pattern of presentation of Pseudomonas aeruginosa in hospital settings. Methods: This cross-sectional study was carried out among 280 specimens which were isolated for a period of 17 months in our tertiary care hospital. The blood culture bottles were placed in Bac T/ Alert 3 D and the positive culture bottle was processed by Grams stain and in routine bacteriological media for inoculation and incubated. Pseudomonas aeruginosa organisms isolated from all the clinical samples were subjected for determining the identification and antibiotic susceptibility profile by VITEK 2 and manual methods. Results: A total of 280 samples were analyzed in this study. The background characteristics of the specimens analyzed is given in table 1. In this study, majority of the samples were from inpatients (71.4%). Among the total samples, most specimens were urine samples (37.5%), followed by pus (23.1%). The organisms showed high sensitivity to Amikacin, Ceftazidime and Gentamycin (98%). Conclusion: From the present findings, we understand that Amikacin and Gentamycin can be the proposed drug of choice for severe infections with Pseudomonas aeruginosa.