This investigation is concentrated on how hematological markers would change in pig fat induced metabolism syndrome (MS) in adult Wistar rats. The objective of the current work was to examine the effect of diet-induced metabolic syndrome on hematological parameters. Metabolic syndrome was induced by feeding rats with diet supplemented to 10% (LF group) and 30% (HF group) pig fat and the control rats were fed normal diet (ND) for 9 weeks. After the study period, White Blood Cell (WBC), Red Blood Cell (RBC), hematocrit (HCT), Hemoglobin, platelet (PLT), lymphocytes, monocytes, Neutrophils, Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV) were measured using BC-30s hematology analyzer. The results showed that hematocrit, RBC and hemoglobin levels were significantly decreased in metabolic syndrome than in control rats respectively (37.24±10.3 vs 33.16±9.5%; 7.08±3.2 vs 6.63±2.8x10 6 /μL and 13.36±1.3 vs 11.85±1.2g/L; p< 0.05). MCHC and platelet were also not elevated in metabolic syndrome than in control rats respectively (31.5±8.2 vs 45.3±10.4 and 356.6±92.2g/dl vs 485.2±89.4x10 6 μL; p<0.05). However, erythrocyte indices as MCV didn't showed statistically significant variations among the LF and control rats (56.08±2.01 vs 55.4±2.8fl; p<0.05). MS appeared to significantly decrease to white blood cell concentration, lymphocytes and neutrophils which probably reduced the immune defense mechanism. The findings demonstrated that MS caused significant undesirable alterations in hematological parameters in rats and it possible to suspect a coexistence between anemia and the metabolic syndrome in this animal model. In conclusion, the rats induced fat metabolism syndrome affected hematocrit and hemoglobin levels and it possible to suspect a coexistence between metabolic syndrome and the anemia in this animal model.
Background:The metabolic syndrome is a state of morbidity characterized by factors such as abdominal obesity, high blood pressure, hyperglycemia, low HDL cholesterol (HDL-C) and high triglyceride levels. The objective of this study was to evaluate the effect of a diet supplemented with pig fat 10% and 30% on the occurrence of at least three of the five factors that characterize metabolic syndrome with a model animal imitating the pathological state of man. Material and methods:The experiment was conducted on three groups of Wistar rats (Rattus norvegicus) ad libitum consumption pig high-fat diet (HF, 30%), pig lowerfat diet (LF, 10%) and normal diet (ND) for 9 weeks. At the end of the experiment, metabolic parameters were obtained and adipose tissue was weighted. Results:The results showed that total cholesterol was significantly increased in rats consuming 10% and 30% fat compared to control rats (142.44±5.3 and 148.86± 4.25 vs 136.02±4.8mg/dl; p < 0.05;n=7). It's the same for blood triglycerides concentration in experimental rats (LF, HF) and control rats with values of 128.25±4.7 and 129.02±5.4 vs 112,4±3.6mg/dl (p<0.05). The LDL cholesterol was singularly high in rats fed the 30% HF diet compared to the rest of the animals (112.8±8.5 vs 96.40±9.3 and 90.4±4.2 mg/dl;p<0.05). The serum fraction of HDL cholesterol was significantly lower in the experimental rats (LF, HF) than the control rats (61±4.9 and 54.97±5.9 vs 93.5±4.1 mg/dl;p<0.05). The atherogenic indices CT/HDL and LDL/HDL were significantly higher in LF and HF rats compared to control rats (1.78 and 1.52 vs 1.34;p<0.05). The plasma glucose level of the experimental rats (LF, HF) was significantly reduced compared to that of the control rats (23.3±3.21 and 25.7±2.4 vs 34.7±3.04mg/dl) (p<0, 05). The percentage of epididymal adipose tissue is also greater in diet animals (HF) than standard (RS) and experimental (LF) rats (1.4±0.30% vs. 0.79±0, 06% and 0.75±0.04%;p <0.05) Conclusion: Elevated levels serum of total cholesterol, LDL cholesterol and atherogenic indices (TC/HDL and LDL/HDL), low HDL-C and excessive accumulation of adipose tissue (abdominal and epidydimal) have shown that the standard diet supplemented with 10% or 30% of pig fat caused a metabolic syndrome. Like the animal fat, incorporation at high doses in the diet could affect health because of the presence of hyperLDLlemia found in our test which one were the main risk factors for cardiovascular disease.
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