Typhonium flagelliforme (Araceae) is a medicinal herb which is endowed with curative properties against a variety of illness including injuries, oedema, coughs, pulmonary ailments, bleeding and cancer. In order to assess its phytochemical components, an experiment was conducted on one to six month old ex vitro and in vitro extracts of T. flagelliforme. The active (ex vitro and in vitro) extracts of T. flagelliforme were screened for phytochemicals components such as alkaloids, flavonlids, terpenoids and steroids. Alkaloids and flavonoids are the main phytochemical constituents of T. flagelliforme which are found to be in the highest amount in two and four month old of ex vitro plants. High amounts of main phytochemical constituents were observed during the flowering process which started in two month old plant and finished at the end of the three month old plant.
In this study, the effects of eyestalk ablation on maturation, moulting, spawning and hatching rate of wild caught Penaeus monodon were investigated. Twenty males (M) and 30 eyestalk-ablated and 30 nonablated females (F), all individually marked (M: F=1:3) were stocked into two 15-ton capacity (each) maturation tank. All the ablated females moulted in 7.92±0.24 (mean±standard error) days, and first spawning occurred 3.2±0.20 days following eyestalk ablation. While twenty-five ablated females spawned, producing an average of 296,160±26,589 eggs, only eight non-ablated females spawned, producing an average of 195,462±20,565 eggs during the investigation period of 120 days. A significant positive correlation between fecundity and female body weight (P<0.05; P<0.001) was observed. Throughout the experiment, the average number of spawning per female was 1.8±0.14 for ablated and one for non-ablated females. Multiple spawning (up to three times) occurred within the same moulting cycle in ablated females. Among the ablated P. monodon females, 36% spawn once, 48% second time, and 16% third time. The average fertility rate of the eggs was high, ranging between 80 and 90% in nonablated females, while in ablated females; the range was between 72 and 88% and differ significantly (p<0.05) ablated and non ablated females. Average hatching rate ranges between 70 and 80% for ablated and 75 and 82% for non-ablated females (p<0.05). From eggs to nauplii production per female was 167,838 for ablated and 127,500for non-ablated females. This study indicates that eyestalk ablation and environmental condition were important inducing tools for re-maturation of spent P. monodon that continued supply of seed stock for effective commercial shrimp farming.
Toxic responses of distilled water, 50% ethyl and absolute ethyl alcohol extracts of six indigenous plants, Datura innoxia (Mill), Clerodendrum viscosum (Vent), Amoora rohituka (Roxb) Wt. et. Arnott, Acacia auriculaeformis A. Cunn. ex. Benth., Pongamia pinnata (L) Pierre and Acacia catechu Willd were studied on a predatory fish Heteropneustes fossilis (Bloch) in laboratory conditions. Amongst the extracts the toxicity range varied, absolute ethyl alcohol extract of A. rohituka seeds being the most toxic (LC 50 = 8.67 ppm) whilst distilled water extract of D. innoxia seeds was the least toxic (LC 50 = 1950.03 ppm). On the basis of LC 50 and relative potency values the relative toxicity of the six plants was in the order-A. rohituka > C. viscosum > P. pinnata > A. auriculaeformis > A. catechu > D. innoxia seed extracts. These six indigenous plant seed extracts assayed might be helpful as controlling agent of undesirable fishes in fish culture ponds.
Haemolytic effects of different extracts of five indigenous plant seeds viz. Datura innoxia (Mill) (Shada Dhutra), Clerodendrum viscosum (Vent.) (Vat), Amoora rohituka (Roxb.) Wt. ct. Arnott (Pitraj), Acacia auriculiformis A. Cunn. Ex. Benth. (Akashmoni) and Pongamia pinnata (L.) Pierre (Karinja) were studied upon a predatory fish, Singhi, Heteropneustes fossilis (Bloch) under laboratory conditions. Toxicants inducing effects on the blood parameters included decreased RBC and WBC counts, low haemoglobin percentage and decreased PCV (packed cell volume), MCV (mean corpuscular volume) and MCH (mean corpuscular haemoglobin). Haemolysis action included shrunken or swollen RBCs, granulated nuclei of RBCs and pyknotic nuclei of WBCs, clumping of RBCs and syncitium of RBC matrix. Damage of blood cells with absolute ethyl alcohol extract was most pronounced with all of the seed extracts. The order of toxicity of the five seed extracts on the blood parameters of Singhi was: D. innoxia < A. auriculaerformis < P. pinnata < C. viscosum < A. rohituka seed extracts. On the basis of haemolysis the toxicity was: A. rohituka > C. viscosum > P. pinnata > D. innoxia > A. auriculiformis seed extracts.
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