The cryptate electrode (Ag/Ag þ 222), prepared by immersing silver wire in a solution of silver(I) salt and the cryptand 222 (4,7,13,16,21,24-hexaoxa-1,10-diazabicyclo[8.8.8]
Polypodium vulgare L., a widely distributed fern, is water-stress tolerant. Under controlled dehydration conditions (20% mannitol, 9 h) without and with abscisic acid (ABA) pretreatment (2 mg l -1 , 24 h), dehydration tolerance and regenerative, potential of common polypody rhizomes was investigated. We demonstrated the positive effect of ABA on changes in dehydrated rhizome metabolism. ABA pretreatment reduced electrolyte leakage from cells: it has also a role in regulating sucrose accumulation and thus, osmotic adjustment. Our findings confirm that P. vulgare rhizomes are well adapted to stress conditions through maintaining of ability to bud formation by dehydrated and rehydrated rhizomes.
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