Objective - Our purpose was to determine the inhibitory effect of methanolic extract of Talinum portulacifolium (METP) left on carbohydrate digestive enzymes, and also establish its activity on glucose uptake in isolated rat hemidiaphragm.Methods To investigate the effect of METP on carbohydrate digestive enzymes we have carried out alpha-glucosidase inhibitory assay by using mouse small intestine homogenate as alpha-glucosidase solution. Also yeast alpha-amylase inhibitory assay was performed. Effect of METP on glucose utilisation was evaluated by estimating glucose uptake in isolated rat hemidiaphragm.Results The METP effectively inhibited alpha-glucosidase and alpha-amylase. In the alpha-amylase inhibitory assay METP (100-1000 µg/ml) showed dose dependent inhibition and standard acarbose (50 µg/ml) showed 50.33% inhibition. METP showed both sucrase and maltase inhibitory activity 86.00 %, 90.66% respectively at 500 µg/ml. The glucose uptake in the hemidiaphragm was significantly increased by METP (17.16 ± 0.20) when compared to the control group. At the same time, uptake was lower than insulin alone (64.2 ± 0.61). The total polyphenolic content of METP was measured and it was 39 µg gallic acid /100 g of fresh weight material.Conclusion These results suggest that METP has significant inhibitory activity on carbohydrate metabolizing enzymes and increases the peripheral utilization of glucose. It may act as a potent antihyperglycemic agent.
The aim of this study is to evaluate antidiabetic and antioxidant effects of methanol extract of Talinum portulacifolium (FORSSK) leaves (Portulacaceae) (METP) in streptozotocin induced diabetic rats. The acute and sub acute antihyperglycemic effect of two different doses (200 and 400 mg/kg,) of METP were investigated. Blood glucose level, serum insulin level, lipid profiles and biomarker enzymes were evaluated in normal and diabetic rats. Liver glycogen content, kidney and liver antioxidant enzymes were evaluated. Supplementation of METP by oral gavage significantly reduces the fasting blood glucose level. This supplementation significantly increases the glycogen level in the liver (32.33±1.453 mg/gm) as compare to diabetic control group (5.66±0.881 mg/gm) (p<0.001). Elevated serum glutamate oxaloacetate transaminase (SGOT), glutamate pyruvate transaminase (SGPT), and alkaline phosphatase (SALP) were decreased significantly by the treatment of METP with respect to diabetic group. The antioxidant activity was studied in liver and kidney tissues by measuring malondialdehyde (MDA), reduced glutathione (GSH) and catalase levels. In the METP treated groups, the HDL/LDL ratio was improved and cholesterol and triglycerides levels also decreased significantly. Histopathological studies of the pancreas of these animals showed comparable regeneration by METP. Glibenclamide was used as a standard at a dose of 0.5 mg/kg p.o
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