New metabolic profiling technologies provide data on a wider range of metabolites than traditional targeted approaches. Metabolomic technologies currently facilitate acquisition of multivariate metabolic data using diverse, mostly hyphenated, chromatographic detection systems, such as GC-MS or liquid chromatography coupled to mass spectrometry, Fourier-transformed infrared spectroscopy or NMR-based methods. Analysis of the resulting data can be performed through a combination of non-supervised and supervised statistical methods, such as independent component analysis and analysis of variance, respectively. These methods reduce the complex data sets to information, which is relevant for the discovery of metabolic markers or for hypothesis-driven, pathway-based analysis. Plant responses to salinity involve changes in the activity of genes and proteins, which invariably lead to changes in plant metabolism. Here, we highlight a selection of recent publications in the salt stress field, and use gas chromatography time-of-flight mass spectrometry profiles of polar fractions from the plant models, Arabidopsis thaliana, Lotus japonicus and Oryza sativa to demonstrate the power of metabolite profiling. We present evidence for conserved and divergent metabolic responses among these three species and conclude that a change in the balance between amino acids and organic acids may be a conserved metabolic response of plants to salt stress.
SummaryTo study the effects of high temperature on the meiosis of pollen mother cells (PMCs) and to determine their relationship with grain set, two in situ experiments were conducted between 2010 and 2012 on four wheat cultivars, Kauz, Montana, M6 and Chamran, under normal (normal cultivation) and terminal heat stress (late cultivation) conditions. Due to the delay in cultivation for the cultivars under stress conditions, their flowering stage faced heat stress at the end of the growing season, and therefore, a significant (p ≤ 0.05) increase was observed in meiotic abnormalities. Cytogenetic studies discovered that the meiosis in PMCs and the pollen development are strongly influenced by heat. Based on the results, abnormalities such as precocious chromosome migration to the poles, laggard chromosomes, micronuclei, absence of metaphase plate, pyknosis, abnormal cytokines, cytomixis and abnormal tetrad were observed in different cultivars. The sensitive cultivars, M6 and Montana, had the greatest percentages of meiotic abnormalities. The meiotic abnormalities showed a significant negative correlation with the number of kernels per spike under terminal heat stress conditions (r = 0.54, n =16, p ≤ 0.01). In other words, due to increasing meiosis abnormalities under terminal heat stress, the grain set was greatly reduced, especially in the susceptible cultivars. The result of this experiment showed that studying the meiosis in PMCs can be suggested as one of the research necessary to improve commercial heat-tolerant cultivars, and to some extent, as a method for screening tolerant lines in breeding programs.
Evaluating physiological traits involved in the response of plants to drought stress could lead us to improve drought tolerance in crops. In this regard, the current project was conducted under a line-source sprinkler irrigation system. Eight commercial bread wheat cultivars {Triticum aestivum L.) were evaluated at four water levels (WL) based on evapotraspiration (ET; WL1, supplying 100% of ET water to WL4, supplying 25% of ET water). Among the cultivars Chamran and Kavir had the highest grain yield at WL4 (4450.7 and 4317.3 kg ha-\ respectively) and noticeable grain yield stability in different water levels. Water use efficiency (WUE) ranged between 5.092 and 7.296 kg ha"m m"^and significantly varied among cultivars in different WLs. Under WL4, Niknejad and Kavir had the highest and Shiraz had the lowest value for WUE. Regression analysis confirmed that the relationship between dry matter and ET follows a linear function. Furthermore, evapotranspiration efficiency (ETE) as calculated by dividing the total biomass by ET was a reliable physiological indicator for cultivar evaluation with regard to water deficit tolerance in different growing stages. Based on this indicator, it was discovered that Mahdavie has the highest ETE from planting to stem elongation, Niknejad from planting to flowering, Pishtaz from planting to dough development and ripening, and Kavir has tolerance against water deficit throughout the growing season.
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