Cell polarity is important for controlling cell shape, motility, and cell division processes. Vimentin intermediate filaments are necessary for proper polarization of migrating fibroblasts and assembly of vimentin and microtubule networks is dynamically coordinated, but the precise details of how vimentin mediates cell polarity remain unclear. Here, we characterize the effects of vimentin on the structure and microtubule-nucleating activity of the cell centrosome and the dynamics of the microtubule network in wild-type and vimentin-null mouse embryonic fibroblasts (mEFs). We find that vimentin mediates the structure of the pericentrosomal material, promotes centrosome-mediated microtubule regrowth, and increases the level of stable acetylated microtubules in the cell. Our results suggest that vimentin modulates centrosome structure and function as well as microtubule network stability, which has importantimplications for how cells establish proper cell polarization and persistent migration.
The ability of bacteria to colonize and grow on different surfaces is an essential process for biofilm development and depends on complex biomechanical interactions between the biofilm and the underlying substrate. Changes in the physical properties of the underlying substrate are known to alter biofilm expansion, but the mechanisms by which biofilms sense and respond to physical features of their environment are still poorly understood. Here, we report the use of synthetic polyacrylamide hydrogels with tunable stiffness and controllable pore size to assess physical effects of the substrate on biofilm development. Using time lapse microscopy to track the growth of expanding Serratia marcescens colonies, we find that biofilm colony growth can increase with increasing substrate stiffness on purely elastic substrates, unlike what is found on traditional agar substrates. Using traction force microscopy, we find that biofilms exert transient stresses correlated over length scales much larger than a single bacterium. Our results are consistent with a model of biofilm development in which the interplay between osmotic pressure arising from the biofilm and the poroelastic response of the underlying substrate controls biofilm growth and morphology.
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