Dencichine (beta-N-oxalyl-l-alpha,beta-diaminopropionic acid) is a haemostatic agent present in well-known traditional Chinese medicinal herbs such as Panax notoginseng, as well as other Panax species. It is also a reported neurotoxic agent found in Lathyrus sativus (grass pea seed) and cycad seeds. A method was developed for quantitative determination of the non-protein amino acid, dencichine, in plant samples of P. notoginseng and the adventitious roots directly from the explants of P. notoginseng after derivatization with ethyl chloroformate (ECF) by gas chromatography-mass spectrometry (GC-MS). l-2-chlorophenylalanine was used as an internal standard. Calibration curves were linear (r(2)=0.9988, n=6) in the range of 10-800 microg/ml for dencichine. Limit of detection and quantification for dencichine were 0.5 microg/ml and 2 microg/ml, respectively. This rapid and specific method may be applied to the quantification of dencichine in complex medicinal plants and their products.
In this paper, rheumatoid arthritis is studied as an example of complex diseases. As we all know, rheumatoid arthritis is a relatively complex disease of autoimmunity. It is caused by genetic and environmental factors. However, the mechanism of its pathogenesis and regulation is still unclear. This paper proposes a new method framework from the system level. We plan to use the already regulated information and functional relationships to explain the risk gene elements. For different risk gene modules, we can use the database to explore the relationship between them and the corresponding transcription factors. I believe that the results obtained will provide useful reference for the development of clinical medicine.
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