Methane (CH 4 ) is a more potent greenhouse gas in terms of climate forcing than carbon dioxide (CO 2 ) (Forster et al., 2007;IPCC, 2018), and in the recent decades, natural sources of CH 4 have accounted for about 22%-30% of mean global CH 4 emissions (Saunois et al., 2020). Although existing estimates and models are updated periodically, our confidence in these remains low because of limited observational data. The importance of global CH 4 models that are based on well-represented estimates from different latitudinal regions has been highlighted in previous studies (Saunois et al., 2020), including studies on freshwaters (Bastviken, Tranvik, et al., 2011).While uncertainties in CH 4 estimates remain, it has been well established in landmark studies that freshwaters are the primary natural sources of atmospheric CH 4 (Bastviken, Tranvik, et al., 2011;Kirschke et al., 2013;Saunois et al., 2020). The most recent global CH 4 emission estimates (2000-2009) from freshwaters, which include lakes, ponds, reservoirs, streams, and rivers, amount to 159 Tg CH 4 yr −1 (Saunois et al., 2020). Different aquatic environments present various and unique challenges in the study of CH 4 dynamics (Hamdan & Wickland, 2016). Most of our current knowledge about CH 4 is derived from boreal and temperate aquatic ecosystems; however, it is expected that different scenarios occur in the tropics. In simulation-based bottom-up estimates by Walter et al. (2001), CH 4 emissions in aquatic ecosystems were found to be sensitive to precipitation and the water table. Nevertheless, that study emphasized that estimates should be updated with representative data from tropical regions.
Food web studies rely heavily on the use of traditional gut content analysis or the fairly popular stable isotope analysis rather than fatty acid analysis (FAA) despite its straightforward process, and ability to identify and characterize more diverse trophic pathways. In this study, we employed fatty acid (FA) biomarkers as a preliminary attempt to trace and characterize trophic pathways in food webs of four tropical lakes of volcanic origin in Luzon Island, Philippines - three clustered maar lakes (Yambo, Pandin and Calibato) and a large caldera lake (Taal). These lakes have a long history of human disturbance but limited ecological data. Knowledge of basal food sources and existing trophic dynamics of organisms are generally non-existent. Particulate organic matter (POM), zooplankton, and fish species were collected in August 2019 from the study lakes as representative of three trophic guilds. Non-metric multidimensional scaling (NMDS) and principal component analysis (PCA) were conducted to analyse FA profiles and characterize trophic relationships between representative organisms. For the POM, within lakes comparison of taxon-specific FA profiles showed a significant difference between the surface and near bottom depths, with the former dominated by photoautorophs and the latter by chemotrophs, suggesting the ability of FAA to effectively delineate between micro-organisms. Between lakes comparison also showed significant difference between the caldera and maar lakes, with the latter containing higher composition of bacterial FA, reminiscent of the considerably smaller lakes’ response to the impact of unmitigated organic loadings from anthropogenic activities. Taken together with the primary consumers’ FA profiles, analysis confirmed the ability of FAA to discriminate between FA profile sources. PCA explained >70% of the variance in the FA compositions for three trophic guilds in the two deepest lakes, which delineated both zooplankton and fish species food selectivity in each lake, alluding to FAA’s capacity to characterize dietary reliance of various species in an environment with numerous food sources. Although certain limitations were encountered, such as the specificity of the sampling depths for POM, and the small sample size of the representative species of the third trophic level, this study demonstrated the effectiveness of FAA as a powerful ecological tool for disentangling intricate lake food webs comprising various food sources. Overall, this study provided baseline information on basal food sources and trophic pathways of representative organisms from four tropical lakes. Taken together, FAA studies have wide application in understanding food webs, including anthropogenically-threatened lake ecosystems.
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