The transcriptional profiles of mouse embryonic, neural, and hematopoietic stem cells were compared to define a genetic program for stem cells. A total of 216 genes are enriched in all three types of stem cells, and several of these genes are clustered in the genome. When compared to differentiated cell types, stem cells express a significantly higher number of genes (represented by expressed sequence tags) whose functions are unknown. Embryonic and neural stem cells have many similarities at the transcriptional level. These results provide a foundation for a more detailed understanding of stem cell biology.
DNA methylation is a heritable epigenetic modification involved in gene silencing, imprinting, and the suppression of retrotransposons1. Global DNA demethylation occurs in the early embryo and the germline2,3 and may be mediated by Tet (ten-eleven-translocation) enzymes4–6, which convert 5-methylcytosine (mC) to 5-hydroxymethylcytosine (hmC)7. Tet enzymes have been extensively studied in mouse embryonic stem cells (ESCs)8–12, which are generally cultured in the absence of Vitamin C (VitC), a potential co-factor for Fe(II) 2-oxoglutarate dioxygenase enzymes like Tets. Here we report that addition of VitC to ESCs promotes Tet activity leading to a rapid and global increase in hmC. This is followed by DNA demethylation of numerous gene promoters and up-regulation of demethylated germline genes. Tet1 binding is enriched near the transcription start site (TSS) of genes affected by VitC treatment. Importantly, VitC, but not other antioxidants, enhances the activity of recombinant Tet1 in a biochemical assay and the VitC-induced changes in hmC and mC are entirely suppressed in Tet1/2 double knockout (Tet DKO) ESCs. VitC has the strongest effects on regions that gain methylation in cultured ESCs compared to blastocysts and in vivo are methylated only after implantation. In contrast, imprinted regions and intracisternal A-particle (IAP) retroelements, which are resistant to demethylation in the early embryo2,13, are resistant to VitC-induced DNA demethylation. Collectively, this study establishes VitC as a direct regulator of Tet activity and DNA methylation fidelity in ESCs.
Summary Transposable elements represent nearly half of mammalian genomes and are generally described as parasites or ‘junk DNA’. The LINE1 retrotransposon is the most abundant class and is thought to be deleterious for cells, yet is paradoxically highly expressed during early development. Here we report that LINE1 plays essential roles in mouse embryonic stem (ES) cells and pre-implantation embryos. In ES cells, LINE1 acts as a nuclear RNA scaffold that recruits Nucleolin and Kap1/Trim28 to repress Dux, the master activator of a transcriptional program specific to the 2-cell embryo. In parallel, LINE1 RNA mediates binding of Nucleolin and Kap1 to rDNA, promoting rRNA synthesis and ES cell self-renewal. In embryos, LINE1 RNA is required for Dux silencing, synthesis of rRNA and exit from the 2-cell stage. The results reveal an essential partnership between LINE1 RNA, Nucleolin, Kap1 and peri-nucleolar chromatin in the regulation of transcription, developmental potency and ES cell self-renewal.
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