The chemical composition, total polyphenols and antioxidant activity in the ethanolic extract of Gentianella dianthoides (Kunth) Fabris. The study was of an experimental type in which the lyophilized extract of Gentianella dianthoides (Kunth) Fabris. The chemical composition was determined by phytochemical screening, it was carried out by color or precipitate formation reactions. The content of total polyphenols was determined colorimetrically using the Folin-Ciocalteu reagent, the gallic acid standard was used at concentrations of 10 to 250 µg/mL. To evaluate the antioxidant activity, it was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction method, in which Trolox® (6-hydroxy-2,5,7,8tetramethylchromic acid) was used as a standard solution. -2-carboxylic) and the lyophilized extract of the sample. Under the experimental conditions it was shown that the lyophilized ethanolic extract of: Gentianella dianthoides (Kunth) Fabris has a yield of 11.73%, the phytochemical test showed abundant content of flavonoids and phenolic compounds, moderate content of anthraquinones and slight content of saponins, the determination of total polyphenols by the Folin -Ciocalteu method showed that the amount of polyphenols is 10.0365 mg/g of extract, the determination of antioxidant activity by the DPPH method, the IC 50 of Trolox® (3.8 µg/mL) and of the ethanolic extract was calculated, which in this case was 27.65 µg/mL. It is concluded that the plant Gentianella dianthoides (Kunth) Fabris presents polyphenolic composition and antioxidant activity.
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