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The scaffold is obtained from acellular bovine bone: Nukbone® (produced by Biocriss SAPI de CV). This acellular bone was subjected to a demineralization process after which the composition was found to be 10% water, 65% of collagen and 25% of hydroxyapatite. The techniques used to characterize these natural scaffolds were: optical microscopy, scanning electron microscopy, X-ray diffraction, energy dispersive X-ray spectroscopy, thermo gravimetric analysis, differential scanning calorimetry and determination of pore size using nitrogen adsorption, and physical adsorption of N2. The pore size is between 100 and 500 microns. These scaffolds have been tested in several biological tissues as urethra, trachea, blood vessels, bone and heart successfully.