We investigated the prooxidant effects of bisphenol A (BPA) phenoxyl radicals in comparison with the phenoxyl radicals of 3-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-methylphenol (BHT) and 4-tert-butylphenol (TBP). The phenoxyl radicals, generated in situ by 1-electron oxidation of the corresponding phenol, were allowed to react with reduced nicotinamide adenine dinucleotide phosphate (NADPH) and rifampicin. The antioxidant activity of various phenols was examined based on the reduction of 2,2'-diphenyl-1-picrylhydrazyl radical (DPPH). It was found that the prooxidant activity of BPA phenoxyl radicals far exceeded those of BHA and BHT of phenoxyl radicals. Unlike Trolox, BPA showed minimal DPPH scavenging activity. The strong prooxidant properties of BPA phenoxyl radicals propelled us to study the markers of cellular oxidative stress in GT1-7 hypothalamic neurons exposed to BPA. It was observed that neuronal cells exposed to BPA had increased generation of intracellular peroxides and mitochondrial superoxide ([Formula: see text]). The formation of peroxides and [Formula: see text] were time- and dose-dependent and that co-incubation with N-acetyl-l-cysteine or Trolox greatly lowered their levels. The results of the present study are consistent with emerging evidence that human populations (non-institutionalized) having higher levels of urinary BPA also have increased levels of oxidative stress markers and are prone to higher risk of cardiovascular diseases, diabetes and abnormalities in hepatic enzymes.
A modified green approach for the synthesis of stable silver nanoparticles (AgNPs) using tea leaf extract is described. The method involves the reduction of silver salt by the polyphenols present in the green tea leaf extract and requires no additional capping/stabilising agents. Compared to other biogenic methods for the synthesis of AgNPs, the uniqueness of the approach described here lies in its simplicity, low-cost, and rapid synthesis rate; the reaction being completed within 10À15 min at room temperature. The reaction was carried out in alkaline medium without stirring and heating, and requires no special cleaning or drying of the glassware used. The synthesised AgNPs were characterised by UVÀVis spectroscopy and transmission electron microscopy (TEM). The results showed that AgNPs with a strong surface plasmon resonance peak around 410 nm and particle size in the 5À30 nm range were prepared. The synthesised AgNPs show excellent chemical stability for more than six months in aqueous solution. Additionally, we showed that the as-synthesised AgNPs can be used as highly selective colorimetric and optical sensors for the detection of cysteine. Thus, with a simple synthesis strategy, and enhanced stability, these green-tea-functionalised AgNPs have the potential for further applications as biosensors and antimicrobial agents.
In view of its potential for developing useful pharamaceutical formulations of furosemide, a widely-used loop diuretic, the crystal structure of the furosemide anion with m-chlorobenzoate has been investigated. In these co-crystals, the monoanions of furosemide and m-chlorobenzoate are balanced by two independent Na+ ions, both of which are hexacoordinated by three monodentate water molecules, two double-water bridge molecules and one single-water bridge molecules, thus yielding centrosymmetric Na2(OH2)8 units linked by single water bridges to form chains in the [10] direction.
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