Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical reactions that employ droplets as microreactors. We developed a novel technology to produce monodisperse emulsion droplets with high efficiency and high throughput using a bench-top centrifuge. Upon centrifugal spinning, the continuous aqueous phase is dispersed into monodisperse droplet jets in air through a micro-channel array (MiCA) and then submerged into oil as a stable emulsion. We performed dPCR reactions with a high dynamic range through the MiCA approach, and demonstrated that this cost-effective method not only eliminates the usage of complex microfluidic devices and control systems, but also greatly suppresses the loss of materials and cross-contamination. MiCA-enabled highly parallel emulsion generation combines both easiness and robustness of picoliter droplet production, and breaks the technical challenges by using conventional lab equipment and supplies.
The coherence of radar echoes is a fundamental observable in interferometric synthetic aperture radar (InSAR) measurements. It provides a quantitative measure of the scattering properties of imaged surfaces and therefore is widely applied to study the physical processes of the Earth. However, unfortunately, the estimated coherence values are often biased due to various reasons such as radar signal nonstationarity and the bias in the estimators used. In this paper, we focus on multitemporal InSAR coherence estimation and present a hybrid approach that mitigates effectively the errors in the estimation. The proposed approach is almost completely self-adaptive and workable for both Gaussian and non-Gaussian SAR scenes. Moreover, the bias of the sample coherence can be mitigated with even only several samples included for a given pixel. Therefore, it is a more pragmatic method for accurate coherence estimation and can be applied actually. Different data sets are used to test the proposed method and demonstrate its advantages.Index Terms-Adaptive hypothesis test, bootstrap, coherence estimation, fringe rate estimation, interferometric synthetic aperture radar (InSAR).
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