The aim of this study was to determine the prevalence of human papillomavirus (HPV) infections in women with different gynaecological diseases (GDs) and ages in southwest China. With the assay of reverse dot blot, a total of 5544 cervical samples, including 1008 normal and 4536 abnormal specimens from women with GDs, were assayed for HPV detection. For the normal group, 20.2% (204/1008) tested positive for HPV, of which 40.2% (82/204) were high-risk (HR-HPV) genotypes. In the 4536 abnormal samples, 1569 women (34.6%) tested positive for HPV: 73.5% (1153/1569) had a single infection, 17.7% (277/1569) dual infection and 8.8% (138/1569) multiple HPV infection. Of 1569 HPV-positive specimens, 58.7% (921/1569) were infected with only HR-HPV genotypes. Significant differences in HPV infections were found among women of different ages (P < 0.01), number of pregnancies (P < 0.01), GDs (P < 0.01) and age at first sex (P < 0.01). In the present study, we found a high prevalence of HPV infection in women with GDs in southwest China. In addition to HPV types 16 and 18, a significant proportion of other HR-HPV genotypes were detected in this population.
Intratypic variations of HPV-18 are known to differ in the persistence of the infection, frequency of carcinogenesis and the progression of precursor lesions to advanced cervical cancer. This study was designed to analyze sequence variations of HPV-18 isolates in order to discover novel HPV-18 variants and to evaluate the variations among infected women in southwest China. Cervical biopsies from 56 HPV-18-positive women with cervical neoplasia were assayed by PCR amplification and sequencing of all eight genes (E1, E2, E4, E5, E6, E7, L1, L2) of the HPV-18 genome. The most frequently observed variation was a C to G transversion at nucleotide 287 of E6, which was found in 48.2% of samples. Analysis of E7 revealed only one specimen as having sequence variations. In addition, we have identified several novel variations: A551C in E6, G6906A in L1, and C4915T and C5147A in L2. The mutations in E6 and L2 are silent, while the E7 mutation results in a single amino acid change. This study complements and expands on previous descriptions of HPV-18 variants. The sequence variation data presented here provides a foundation for future research on HPV-induced oncogenesis and may prove valuable for developing diagnostic probes and in the design of HPV vaccines for targeted populations.
Background: A 38-year-old woman conceived twins by in-vitro fertilization (IVF). Ultrasound examinations showed signs of monochorionic twins.Case presentation: Standard karyotyping of peripheral blood cells from the twins showed chimerism 47,XY,+21/46,XX, mainly 46,XX line. Single nucleotide polymorphism microarray (SNP-array) analysis of buccal membrane cells from the twins showed a single cell line 46,XX in the twin girl and a single cell line 47,XY,+21 in the twin boy. Three-year follow-up after the birth showed that the twin girl had normal development, while the twin boy had Down Syndrome.Conclusion: This is the first report on monochorionic dizygotic (MCDZ) twins by IVF with normal development/Down Syndrome respectively. To evaluate prenatal diagnosis of twins, early determination of chorionic status is important, and the amniotic fluid karyotyping is preferred rather than chorion villus sampling (CVS) or cord blood. It is important to perform genetic analysis of different tissues of MCDZ twins. Future research is needed to identify the causes and long-term consequences of chimerism in twins.
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