Simple SummaryOld, sick, and low-production cows are called cull cows, and they are sent from the farms to slaughter to produce meat. Cull cows may be more vulnerable than other categories of cattle regarding alterations of their welfare, but most studies linking animal welfare to meat quality have been carried out in steers and heifers of higher commercial value. In this study, we registered health of cull cows at arrival at a slaughterhouse and handling of the cows during the stunning process and associated those variables with carcass bruising and condemnations; the latter are a reflection of the treatment received by the animals before death and therefore can be used as indicators of animal welfare. We found a high percentage of cull cows arriving with low body condition (skinny), as well as presenting lameness and mastitis. During the stunning process 16% of cows did not fall unconscious after the first shot with the penetrating captive bolt gun. Skinny cows and those with mastitis were more likely to be condemned; skinny cows also presented more severe bruises. We conclude that for cull cows the main animal welfare issue originates at farm level, and cows should be culled sooner.AbstractThe objective of this study was to evaluate the welfare of cull cows in a slaughtering plant, using indicators of health on arrival and indicators of handling during the stunning process. These pre-slaughter indicators were associated with post-slaughter indicators of the same cows, such as carcass bruising and condemnations. Transport staff surveys showed that all drivers had been trained on animal welfare. All loads of cows came directly from farms and had an average transport duration of 5 h 22 min. Indicators were registered in 237 cows during unloading at the slaughterhouse and in the stunning box. Bruises and condemnations were recorded post-slaughter in the carcasses of the same cows. Results at arrival showed that 48% of the cows had low body condition, 50% had mammary problems, and 24% suffered from lameness. During stunning, 16% of cows needed a second shot, and 54% exceeded the 60 s established as a recommended interval between stunning and bleeding. During the post-slaughter evaluation, 50% of the carcasses had more than two bruises and 70.46% had a bruise severity score different from zero. Low body condition was a risk factor to increase the severity of bruises; low body condition and mammary problems increased carcass condemnations; the stunning process indicators were not statistically associated with the severity of the bruises. For cull cows the main animal welfare issue originates at farm level.

Even though hemotrophic mycoplasma (hemoplasma) infections are well documented in a wide variety of hosts worldwide, there is a gap in the knowledge aobut hemoplasmas in rodents. This study aimed to molecularly survey and investigate the genetic diversity of hemoplasmas in rodents from Chile. Synanthropic and wild rodents (n = 74) were captured in the southern province of Valdivia (Corral, Valdivia, Riñihue, and Reumén localities). Spleen samples were submitted to a conventional PCR for hemotrophic Mycoplasma spp. targeting the 16S rRNA gene (800 bp), followed by sequencing, phylogenetic, and genetic diversity analyses. The overall occurrence of hemotrophic mycoplasmas in rodents from Valdivia was 24.5% (18/74) [95% CI (14.5; 34.1)]. Hemoplasmas were detected in Mus musculus (1/4), Rattus norvegicus (1/16), Abrothrix longipilis (7/13), A. olivaceo (6/8), and Oligoryzomys longicaudatus (3/10). The nucleotide polymorphism analysis of the targeted 16S rRNA region showed low diversity, with two genotypes and a high identity to the variants detected in wild rodents from Brazil. Hemoplasmas are described for the first time in rodents from Chile with a moderate occurrence and low 16S rDNA genetic diversity within the sampled rodent population. The detected hemoplasma genotypes were specific to rodents and were not shared with other mammals.

The objective of this study was to evaluate the effects of dietary brown seaweed (Macrocystis pyrifera) additive (SWA) on meat quality and nutrient composition of commercial fattening pigs. The treatments were: Regular diet with 0% inclusion of SWA (CON); Regular diet with 2% SWA (2%-SWA); Regular diet with 4% SWA (4%-SWA). After slaughtering, five carcasses from each group were selected, and longissimus lumborum (LL) samples were taken for meat quality and chemical composition analysis. Meat quality traits (except redness intensity) were not affected (p > 0.05) by treatments. Samples from the 4%-SWA treatment showed the lowest a value than those from the 2%-SWA and CON treatments (p = 0.05). Meat samples from the 4%-SWA group contained 3.37 and 3.81 mg/100 g more of muscle cholesterol than CON and 2% SWA groups, respectively (p < 0.05). The SWA treatments affected (p ≤ 0.05) the content of ash, Mn, Fe, and Cu. The LL samples from 4%-SWA had the highest content of ash; however, they showed 0.13, 0.45, and 0.23 less mg/100 g of Mn, Fe, and Zn, respectively, compared to samples from CON (p ≤ 0.05). Fatty acids composition and macro minerals content (Na, Mg, and K) did not show variation due to the SWA treatments. Further studies are needed to understand the biological effects of these components on adipogenesis, cholesterol metabolism, and mineral deposition in muscle.

The welfare status of cull cows going through livestock markets was assessed in 12 premises in Chile, using behavioral and health indicators observed during unloading, auction, and loading (once in winter and once in summer). Groups of cows were observed by the same observer and the following indicators were recorded at each stage: slips, falls, balks, turns, jumps, and vocalizations of cows were considered as behavioral events and a proportion was calculated based on the number of observed events per group divided by the number of cows per group to give a behavioral event index (BEI). Health status of the cows was assessed during auction by recording the proportion of cows with low body condition, lameness, udder problems, tegumentary lesions, and tail abnormalities. Handler behavior was assessed using a count of negative tactile interactions (NTI) with the cows, like blows/hits, kicks, and pokes with the devices used to drive them, then a NTI index (NTII) was calculated as for BEI. Using the groups of cows as the statistical unit, statistical models were built and used to identify how NTII, some facilities features and comingling were associated with BEI registered during unloading, auction, and loading and also to calculate if the selling price was associated with the different health problems of cows, using the software MLwiN 3.03. A total of 1,103 groups of cows (n = 3,963 cows) were observed, finding a high percentage of slips and balks, whereas hitting and poking were frequent NTI. The highest mean BEI was observed during unloading in winter (1.10), whilst the lowest one was found during auction in summer (0.34). There was an increase of 0.11 in BEI for every extra unit increase in the NTII by the handlers. The BEI was negatively affected by the winter season compared to summer. Of 1,608 cows, 49.8% had a low body condition, 28.3% had udder problems, 24% were lame, 8.7% presented tegumentary lesions, and 3.1% tail abnormalities. It can be concluded that the health of the cull cows is already compromised when leaving the farms; cow behavior and handler tactile interactions with the cows are useful indicators to assess the welfare of cull cows at livestock markets.