Labour Market Intermediaries (LMIs) with a social purpose, or Labour Market Social Innovation, are bubbling since the 1980s due to the fragmentation of labour standards and the growing number of “non-standard forms of work”. However, they face an important lack of legitimacy especially in countries like France where labour market is highly regulated. Analysing the legitimation trajectories two profit-limited LMIs, the Employers’ Associations and the Business and the Employment Cooperatives in France, we highlight the role of meta-organisations and some key factors of success and failure.
Si les bienfaits de la confiance dans les relations de collaboration font l’objet de nombreuses études, ses effets indésirables sont peu traités. En questionnant le processus d’institutionnalisation de la confiance et notamment les mécanismes de reproduction automatique de la confiance qui en résultent, l’étude de cas tend à pallier ce manque. à partir d’entretiens et d’analyses documentaires, les auteurs retracent l’histoire d’une coopérative d’activité et d’emploi, et mettent en lumière le processus de construction d’un excès de confiance. Dans leur cas, le contexte dans lequel la relation de collaboration voit le jour est caractérisé par une prégnance des mécanismes informels et une forte imbrication des acteurs. Ceci facilite l’apparition de biais cognitifs agissant sur le processus d’institutionnalisation de la confiance et débouchant sur un excès de confiance.
S. pneumoniae is the leading cause of community-acquired infections worldwide. Its global success might in part be explained by its genetic transformation, which consists of the internalization and the incorporation in the bacterial chromosome of exogenous DNA. Transformation is turned on in cells which are in a physiological state called competence. The induction of the competent state depends on the two-component regulatory system ComD-ComE. The response regulator (RR) ComE is phosphorylated by the histidine-kinase ComD, and acts as transcriptional activator of a large set of competencespecific operons. These genes encode in particular proteins involved in binding, uptake, processing, and recombination of exogenous DNA, but they are also involved in the virulence of S. pneumoniae 1 . The prototypical RR contains a conserved regulatory domain, linked to a variable effector domain. The majority of RRs (63%) contain DNA binding effector domains. Among them, 5% interact with DNA through the unusual and recently characterized LytTR domain 2 . They form the AgrA/LytR RR family and regulate production of many important virulence factors. Despite their interest for drug development, no full-length RR structure has been determined yet within this family. ComE belongs to the AgrA/LytR family. We initiated a structural study of the ComE non-phosphorylable D58A mutant, and solved its X-ray 3D structure at 3.4A resolution. As expected, ComE D58A contains a conserved regulatory domain and a LytTR domain. The two domains are linked by a long non-structured linker. Interestingly, ComE D58A forms a dimer in the crystal. The regulatory domains are linked by a two-fold symmetry axis within the dimer, whereas the LytTR domains are related by both a translation and rotation (head-to-tail). This asymmetric dimer configuration is facilitated by the flexibility of the linker. The sequence of the ComE promoter is known and consists of two direct repeats. Although ComE D58A was crystallized without DNA, the dimer conformation seems to be consistent with promoter binding, which incited us to set out for a functional mechanistic study. It is well-known that if single crystals of sufficient size are available, single crystal X-ray diffractometry is used for structure solution. Else, structure of the new phase should be solved by other methods. In the era of nano-science, standard powder X-ray diffraction is not always applicable due to the peak broadening effect, thus electron crystallography (EC) emerges as important and sometimes the only possible tool for structure determination of nano-sized crystals. One of the essential problems causing a limited use of EC for this purpose is the dynamical nature of electron scattering. In the past decade, researchers have shown that influence of dynamical effects on the intensities of diffracted beams can be substantially reduced if, instead of conventional illumination of the specimen, beam precession technique (PED) is used [1], [2]. Following this finding, several structures of zeolites, complex...
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