Guanine-rich DNA sequences self-assemble into highly stable fourfold structures known as DNA-quadruplexes (or G-quadruplexes). G-quadruplexes have furthermore the tendency to associate into one-dimensional supramolecular aggregates termed G-wires. We studied the formation of G-wires in solutions of the sequences d(G4C2)n with n = 1, 2, and 4. The d(G4C2)n repeats, which are associated with some fatal neurological disorders, especially amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), represent a challenging research topic due to their extensive structural polymorphism. We used dynamic light scattering (DLS) to measure translational diffusion coefficients and consequently resolve the length of the larger aggregates formed in solution. We found that all three sequences assemble into longer structures than previously reported. The d(G4C2) formed extremely long G-wires with lengths beyond 80 nm. The d(G4C2)2 formed a relatively short stacked dimeric quadruplex, while d(G4C2)4 formed multimers corresponding to seven stacked intramolecular quadruplexes. Profound differences between the multimerization properties of the investigated sequences were also confirmed by the AFM imaging of surface films. We propose that π-π stacking of the basic G-quadruplex units plays a vital role in the multimerization mechanism, which might be relevant for transformation from the regular medium-length to disease-related long d(G4C2)n repeats.
This article presents an undergraduate project for measuring the speed of sound in the air as a function of air temperature. The measurement is implemented in two versions. The simplicity of the basic experiment in dry air is used for a detailed analysis of systematic errors: the measured values are corrected with an additive constant and optimized to match the expected parabolic dependence of the speed of sound on temperature. Such a time correction is correlated with the physical content, that is, by changing the phase of oscillation of the pressure difference at the reflection of the sound at the open end of the tube. The second part of the experiment involves measuring the speed of sound in saturated humid air as a function of air temperature and allows for a substantive connection of two areas of physics (waves and thermodynamics). The measured water vapour pressure, within the range of statistical errors, follows the theoretical course up to the upper limit of the measured temperature range (60℃) surprisingly well.
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