The Aim: To evaluate the association of TSHR gene polymorphism rs2268458 with some Physio-biochemical parameters among hypothyroidism women.
Methods: This study included 51 hypothyroidism women and 43 healthy women as a control group. Some Physio-biochemical (body mass index (BMI), cytochrome 450 1A1 (CYP1A1), calcium, and phosphorus) and hormonal assay (Osteopontin (OPN), thyroid stimulating hormone (TSH), fT3, fT4 and Calcitonin (CT)) assay were performed. Genotyping of rs-2268458 of TSHR gene was carried out using the polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) technique.
Results: TSHR rs2268458 was associated with hypothyroidism in Iraqi women and allele C can be a risk factor for disease by OR= 4.393, CI=1.42-13.53 where TC and CC genotypes were associated with high BMI, while TC genotype was associated with high calcitonin and CYP1A1 levels.
Conclusion: The TSHR rs2268458 polymorphism was associated with hypothyroidism in Iraqi women and allele C can be a risk factor for some Physio-biochemical and hormonal disorder in hypothyroidism women.
The current study was designed to investigate the alterations in the ultrastructure of orgenelles and cellular activity of exocrine pancreatic acini of experimentally induced-diabetic rats and to assess the usefulness of herbal combination supplementation in improving the ultrastructure and cellular activity of exocrine pancreas. The number of albino male rats used were 24 which divided into equally 4 groups; group I: control group, group II: alloxan-induced diabetes mellitus (single intraperitoneal dose of alloxan 120 mg/kg for 3 days), group III: herbal combination treatment composed from the extracts of fenugreek seeds (Trigonella foenum-graecum), black cumin (Nigella sativa) seeds, rhizomes of ginger (Zingiber officinale), leaves of olive (Olea europeae), and seeds of ash (Fraxinus ssp). Each rat given 2.5 ml (0.5 ml from each five mixed plant extracts used) for 2 months. group IV: diabetes was induced as group II and treated with herbal combination as group III for 2 months. The processing for investigating by transmission electron microscopy were carried out for all pancreata taken from all groups. There was significant reduction (p<0.05) in mean of FBG levels in diabetic rats treated with herbal combination (group IV) as compared to diabetic control and could revert FBG to normal value compared to negative control The results of examination of semithin and ultrathin sections in diabetic rats revealed many degenerative changes in the exocrine pancreatic tissue in comparison to control group. These degenerative changes can be summarized as disturbances in the arrangement of pancreatic acinar cells and decreased secretory granules in addition to the vacuolation of cytoplasm and pyknotic nuclei were observed in semithin sections. Moreover, degenerated nuclei, vacuolation of cytoplasm, fragmentation of rough endoplasm reticulum and degranulation of the most pancreatic acinar cells were noticed in ultrathin sections. In contrast, the acinar cells of group administrated with herbal combination only had normal ultrastructure, cellular activity, and nuclei, well-developed rough endoplasmic reticulum with abundant zymogen granules. Also, most of the acinar cells retrieved their normal ultrastructure and cellular activity in diabetic group treated with herbal combination and herbal combination could decrease most of the degenerative changes caused by alloxan-induced diabetes. In conclusion, the daily supplementation with this herbal combination to diabetic rat for 2 months could achieving promising effects due to its cytoprotective influence and showed the ability to decreased degenerative damage in the ultrastructure and cellular activity of exocrine pancreatic acini in type 2 diabetic rats.
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