During the period from December 2014 to July 2015, isolation and identification of pathogenic bacteria from clinical sources attending to AL-Hakeem Hospital and AL-Sadder Medical City in AL-Najaf province. A total of One hundred clinical samples from various sources included: UTI - patients, Burn, postoperative surgery site –Wound, The results revealed the occurrence (52) bacterial isolate were isolated, 37(71%) were gram– positive bacteria that was divided into S. aureus 20(39%), S. pyogen 9 (17%), and S. pneumonia 8 (15%). But only 15 (29%) was gram – negative bacteria which represented by E. coli 7(13%), and P.aeruginasa 4 (8%), Protuse mirabilis 4(8%). The susceptibility of S. aureus and E.coli bacteria which are consider the most common G+ve and G-ve nosocomial pathogen to routenly used antibiotic in Najaf hospitals were tested by using antibiotic susceptibility pattrons, in order to select one isolate from S.aureus and other one from E.coli which had the higher resistance to most antibiotic for continue the other steps of these study. The result illustrated that S.aureus No.5 and E. coli No.3 were greatly resistant to antibiotics, so regard as multi-drug resistant(MDR). Crud extracts of Linum usitatissimum which had been extracted by three solvents (petroleum ether, hexan and aqueous), for studying the chemical contents of these extracts by using suitable reagents and tested antibacterial activity on the growth of studied S.aureus and E. coli bacteria. The extraction of Essential fatty acid Semicarbazide (omega-3-6- fatty acid) from Linum usitatissium (Flax seed) and the chemical and physical properties of isolated oil compounds were studied by using thin layer chromatography (TLC), ultraviolet spectrum (UV) and FTIR spectrum. The result revealed that shown high antibacterial activity against studied (S.aureus, E.coli). results explained broad spectrum antibacterial property of oil compounds against studied bacteria nosocomial.
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