Flow cytometric immunophenotypic analysis (FCA) can be performed to evaluate lymphoid cells in cerebrospinal fluid (CSF). We compared this method with conventional cytologic diagnosis to determine its utility. A retrospective comparison of 35 consecutive CSF flow cytometry results with the corresponding cytologic diagnoses was undertaken. Twenty‐five of 35 CSFs (71%) were successfully analyzed by flow cytometry. The 10 samples which could not be analyzed were either too old (greater than 3 days) or had an insufficient number of cells. A total of 9 lymphomas was detected: 4 by both flow cytometry and cytology; 2 by cytology alone; and 3 by flow cytometry alone. This represents a 50% increase in the detection of lymphoproliferative disorders in CSF by a combination of flow cytometry and cytology vs. cytology alone. Furthermore, in 3 cases with follow‐up where the cytologic diagnosis was “atypical cells of undetermined significance” and the flow cytometric findings were negative for malignancy, the clinical course confirmed a benign pleocytosis in all three. We conclude that flow cytometric analysis markedly improves sensitivity when used in combination with cytology in the evaluation of lymphoid cells in CSF. Diagn. Cytopathol. 2000;23:369–374. © 2000 Wiley‐Liss, Inc.
Background: ß thalassemia is one of commonest inherited disorder in Pakistan having a carrier rate of 5%. Pregnant women having ß thalassemia trait can have offspring having ß thalassemia major which is lifelong blood transfusion dependent state. Different screening programmes are being carried out in various parts of the world so that the birth of children having ß thalassemia major can be effectively reduced.Objective: To determine the diagnostic accuracy of hematological parameters in identifying β thalassemia trait in pregnant women keeping measurement of HgbA2 as gold standard.Methods: 108 pregnant women were enrolled during one year of the study period. Blood sample was taken and blood complete picture was obtained. By using variables of CP card Green & King index, Shine & Lal index and RDWI were calculated keeping hemoglobin electrophoresis as gold standard. Results:The age of patients ranged from 17 to 37 years with mean age of 24.65. Out of 108 pregnant women enrolled in study 40 were found to have ß thalassemia trait based on results of hemoglobin electrophoresis. Shine & Lal index showed 100% sensitivity, 0% specificity, 35% PPV and 0% NPV. Green & Kings index had sensitivity of 32%, specificity 100%, PPV of 100% and NPV of 71% while RDWI showed 50% sensitivity, 100% specificity, PPV of 100% and NPV of 77% in diagnosing ß thalassemia trait in pregnant women. Conclusion:Automated cell counters based formula including Green & King index, Shine &Lal index and RDWI provide rapid, reliable and cost effective method for screening of ß thalassemia trait especially in third world countries like Pakistan. However none of them have 100% sensitivity and specificity. So they should be collectively looked at in screening programmes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.